SFEIES24 Poster Presentations Adrenal & Cardiovascular (40 abstracts)
Uptake of K20-coated iron oxide nanoparticles by adrenocortical carcinoma cells
Ritihaas Surya Challapalli 1 , Anna Sorushanova 1 , Cong Hong 1 , Nathan Mullen 1 , Sarah Feely 1 , Obdulia Covarrubias-Zambrano 2 , Jose Covarrubias 2 , Sunitha Varghese 2 , Constanza Hantel 3,4 , Peter Owens 1 , Martin O’Halloran 1 , Punit Prakash 5 , Stefan Bossmann 2 & Michael Conall Dennedy 1
1University of Galway, Galway, Ireland; 2The University of Kansas Medical Center, Kansas, USA; 3University Hospital Zurich, Zurich, Switzerland; 4University Hopsital Carl Gustav Carus Dresden, Dresden, Germany; 5Kansas State University, Kansas, USA
Adrenocortical carcinoma (ACC) is a rare cancer with a poor prognosis, typically resulting in a survival rate of up to 24 months. Treatment options include surgery, chemotherapy, and radiation, but the high recurrence rate underscores the need for new therapies. Magnetic iron oxide nanoparticles (IONP) are emerging as promising tools for cancer treatment due to their customizable size, shape, and surface properties for targeted cellular uptake. Our initial study investigated the uptake of IONP by ACC cells (H295R, HAC15, and MUC1), HUVECs, and primary monocytes, after incubation with 0, 5, 10, 20, and 50 µg/ml of IONP for 24 hours. The results indicated that nanoparticle uptake was both concentration and time-dependent and affected cellular viability. The optimal IONP concentration was found to be 10 µg/ml, which was then used to examine the uptake rate and intracellular distribution. Importantly, IONP did not affect metabolic respiration or steroidogenesis when ACCs were stimulated. The uptake efficiency of IONP by ACC cells decreased when primary monocytes and an HUVEC layer were present, as shown by a Transwell migration system. This study highlighted that ACC cells exhibit non-specific IONP uptake primarily through macropinocytosis. These initial findings provide a basis for future research aimed at enhancing specific uptake by ACC cells, potentially improving applications like magnet-induced thermal ablation or nanobiocontrast.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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