Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2024) 101 PS3-20-09 | DOI: 10.1530/endoabs.101.PS2-20-09

ETA2024 Poster Presentations Thyroid function, feedback & disruptors (9 abstracts)

Identification of markers for thyroid signaling disruption by transcriptomic analysis in teleost fish and amphibian

Laurent Sachs 1 , Alicia Tribondeau 2 , Célia Marti-Martinez 3 , Corine Blugeon 4 , Noémie De Croze 5 , Marc Léonard 5 , David Du Pasquier 6 & Nicolas Buisine 7


1Muséum National D’histoire Naturelle - Cnrs, Umr 7221 Molecular Physiology and Adaption, Molecular Physiologie and Adaption, Paris, France; 2Muséum National D’histoire Naturelle - Cnrs; 3Muséum National D’histoire Naturelle - Cnrs - L’oréal Research & Innovation; 4Institut de Biologie de L’école Normale Supérieure de Paris, Génomique Ens; 5L’oréal Research & Innovation; 6Watchfrog Laboratory; 7Muséum National D’histoire Naturelle, Centre National de la Recherche Scientifique, Paris, France


Environmental pollution is a major concern in households and ecosystems, many of which interfere with the homeostatic balance of living systems. Endocrine disruptors affect many biological pathways including thyroid axis. The identification of Thyroid Disruptor Chemicals and of their effects on organisms is a challenge for both regulators and industry. As thyroid signaling is highly conserved in vertebrates, zebrafish and amphibian embryos are alternative models for studying both physiological regulations and disruption. We designed a transcriptomic analysis where embryos of both species are exposed to reference compounds alone or in combination with thyroid hormones (T3 and T4) following the Fish Embryo Test (OECD guideline, No.236) or the Xenopus Eleutheroembryonic Thyroid Assay (OECD guideline, No.248). We selected four reference compounds: Iopanoic acid, Sodium Perchlorate, Tetrabromobisphenol A and Propylthiouracil based on their different modes of action on the thyroid signaling pathway. We analyzed their transcriptomes by RNA sequencing together with ad hoc bioinformatic pipelines. Lists of differentially regulated genes are classified in several clusters, each corresponding to a type of biological response. Despite a great diversity of biological responses, differentially regulated genes can be further classified into three categories: solely chemical dependent, solely Thyroid Hormone dependent and crosstalk responses. We first found major effects with chemicals alone, but virtually no effect with THs alone in zebrafish and the opposite in Xenopus. Second, co-treatments revealed for both species potential thyroid signaling disruption. The crosstalk responses represented a significant number of patterns in all conditions. The biological processes affected are related to metabolism (mainly related to lipid and carboxylic acid metabolism) in zebrafish and cell proliferation (covering cell cycle, DNA repair and cell division processes) in Xenopus. Stratification of the datasets with system biology uncovered additional processes affected linked to nervous and immune systems. Finally, we provide a list of candidate thyroid signaling disruption markers. In conclusion, our data provide a set of genes that represent a potential list of biomarkers of thyroid signaling disruption, and the associated effects.

Volume 101

46th Annual Meeting of the European Thyroid Association (ETA) 2024

European Thyroid Association 

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