ETA2024 Poster Presentations Translational thyroid cancer research-1 (10 abstracts)
Usefulness of metabolomic analyses in the pre surgical evaluation of thyroid nodules
Fabio Maino 1 , Silvia Cantara 2 , Elisa Mattii 3 , Daniela Grasso 3 , Andrea Bernini 3 , Antonia Salvemini 3 , Virginia Mancini 3 & Maria Grazia Castagna 4
1University of Siena, Department of Medical, Surgical and Neurological Sciences, Siena, Italy; 2University of Siena, U.O.C. Endocrinologia, Dep. of Surgical, Medical and Neurol, Siena, Italy; 3University of Siena; 4University of Siena, Dsmcn, Siena, Italy
Objectives: Metabolomic is a newly emerging technology with great diagnostic potential in the oncology field. Several studies have evaluated the usefulness of the metabolomic approach in the diagnosis of differentiated thyroid carcinoma (DTC), but most of them analyzed ex vivo samples. High-field nuclear magnetic resonance (NMR), a versatile and quantitative technique, allows the analysis of complex mixtures with minimal sample preparation, identifying molecules in a wide range of molecular weights. The aim of our study was to evaluated the feasibility of metabolomic’s approach in vivo samples and to identify those metabolites able to discriminate between benign and malignant nodules.
Methods: we selected 41 samples of thyroid nodules submitted to fine needle aspiration (FNAC) preserving a minimum portion for NMR analysis. Twentythree/41 (56.1%) were classified Thy2, while 18/41 (43.9%) Thy5.
Results: The multivariate analysis between benign and malignant samples highlighted a significant differentiation of the two groups, with a lower relative concentration of citrate, a metabolite already associated with the progression and invasiveness of tumors, which was 8 times lower in the Thy5 compared to Thy2 (P = 0.0002). Another significant lower concentration of metabolites in Thy5 was observed for serine (P = 0.02), while creatinine was significantly higher (P = 0.03).
Conclusions: These data demonstrates the usefulness and feasibility of using high-field NMR in the analysis of thyroid samples in vivo, identifying and quantifying 60 metabolites, and indicating citrate as the most discriminating metabolite between benign and malignant nodules. These results, although preliminary, could play a crucial role especially in the diagnosis of nodules with indeterminate cytology, selecting more accurately those patients to be sent for surgery.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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