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Endocrine Abstracts (2024) 99 P428 | DOI: 10.1530/endoabs.99.P428

ECE2024 Poster Presentations Adrenal and Cardiovascular Endocrinology (95 abstracts)

Development and validation of LC–MS/MS assay for simultaneous determination of thirteen steroid hormones and two synthetic steroids in saliva: potential utility for pediatric population and beyond

Sandra R Dahl , Liv Hanne Bakke , Per Medboe Thorsby & Svetlana Zykova


Hormone Laboratory, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway


Background: Saliva samples offer the possibility to obtain stress-free non-invasive samples, also for home-testing, especially useful when blood collection is either undesirable or difficult.The aim of this work was to develop an LC–MS/MS method for the determination of clinically relevant steroid hormones cortisol, cortisone, 11-deoxycortisol, 21-deoxycortisol, 17OH-progesterone, aldosterone, corticosterone, deoxycorticosterone, betamethasone, dexamethasone, testosterone, androstenedione, DHEAS, DHEA and 17OH-pregnenolone. A special effort was made to adopt the method to neonatal population with respect to low sample volumes and multiplexing.

Results: Ten microliters were acceptable but 50 μl preferable sample volume for all the analytes. Cortisol, cortisone, aldosterone, 11-deoxycortisol, deoxycorticosterone, dexamethasone, betamethasone, 17OH-pregnenolone and DHEAS could be determined in as little as 2–5 μl saliva. Total analytical variation was <15%, except for 17OH-progesterone, deoxycorticosterone, 17OH-pregnenolone, 21-deoxycortisol, androstenedione, DHEA and betamethasone, that could only be determined semi-quantitatively or qualitatively. The minimum turnaround time is 3–4 h. Recovery from the best-performing sample collection swab SalivaBio ranged from 83 to 127%. Aldosterone, cortisone and DHEA were more stable in saliva compared to serum when stored at ambient temperature for one week. Corticosterone and 17OH-progesterone needed immediate freezing.

Conclusion: The non-invasiveness, small saliva volume requirement, on-swab stability and analytical performance make the method relevant for both research and diagnostics, above all in the setting of neonatal intensive care unit.

Volume 99

26th European Congress of Endocrinology

Stockholm, Sweden
11 May 2024 - 14 May 2024

European Society of Endocrinology 

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