ECE2024 Poster Presentations Thyroid (58 abstracts)
Serum CD5L as potential biomarker of thyroid hormone status during pregnancy
Sabrina Asaad 1 , Thilo Chillon 1 , Dorota Filipowicz 2 , Britta Wilms 3 , Frank Strenge 4 , 5 , Ewelina Szczepanek-Parulska 2 , Minich Waldemar 1 , Sebastian Meyhöfer 6 , 7 , Jens Marquardt 5 , Jens Mittag 3 , Henrik Oster 4 , Marek Ruchala 2 & Lutz Schomburg 1
1The Institute for Experimental Endocrinology, Charité-Universitätsmedizin; 2Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences; 3Institute for Endocrinology and Diabetes, Center of Brain Behavior & Metabolism, University of Lübeck; 4Institute of Neurobiology, Center of Brain, Behavior and Metabolism, University of Lübeck; 5Department of Medicine I, University of Lübeck; 6Institute for Endocrinology & Diabetes, University of Lübeck; 7German Center for Diabetes Research (DZD)
Introduction: The thyroid hormone (TH) status is routinely assessed by thyrotropin (TSH) and thyroxine (T4). Both biomarkers are mainly regulated by thyroid hormone receptor beta, whereas many peripheral organs employ the alpha receptor. Serum CD5L is a liver-derived protein under control of both TH receptor isoforms, but the data base is small [1]. An additional biomarker of TH status is needed in particular during pregnancy, where the routine biomarkers become dynamically disturbed [2]. Ideally, such a diagnostic parameter should display little circadian variation in order to be suitable for routine measurements [3].
Objective: This study aimed to determine possible covariates regulating serum CD5L and to test its potential suitability as additional TH biomarker during pregnancy.
Subjects and Methods: A sandwich ELISA for serum CD5L was established using newly raised antibodies. Circadian effects and the impact of liver disease on serum CD5L concentrations were assessed. Serum samples from pregnant women with well-characterized TH and trace element status were analyzed, and CD5L concentrations were correlated with other indicators of TH status including TSH, fT4, fT3, copper and selenium concentrations.
Results: The new quantitative assay for CD5L showed high accuracy. Serum CD5L was stable in dilution and refreezing experiments and did not show strong circadian variance or dependency on liver disease. In serum of pregnant women, CD5L correlated positively to fT3, but not to fT4 or TSH. Significant positive correlations of CD5L were observed with serum levels of the TH-responsive trace elements selenium and copper.
Conclusion: The data support the potential suitability of serum CD5L as an additional marker of TH status, with potential value for pregnancy and thyroid disease. This notion needs to be tested in sufficiently large clinical studies.
References: 1 Nock S et al. 2020. CD5L Constitutes a Novel Biomarker for Integrated Hepatic Thyroid Hormone Action. Thyroid. 30: 908-923. 2 Osinga JAJ et al. 2022. TSH and FT4 Reference Intervals in Pregnancy: A Systematic Review and Individual Participant. J Clin Endocrinol Metab. 107(10): 2925-2933. 3 M Astiz, I Heyde, and H Oster. 2019. Mechanisms of Communication in the Mammalian Circadian Timing System. Int J Mol Sci. 20(2): 343.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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