ECE2024 Eposter Presentations Adrenal and Cardiovascular Endocrinology (155 abstracts)
Low sodium diet affects microbiome and immunophenotype in patients with primary aldosteronism in a sex-dependent manner
Hanna F. Nowotny 1 , Tingting Zheng 2 , Thomas Marchant Seiter 1 , Jing Ju 1 , Holger Schneider 1 , Kroiss Matthias 1 , Anna-Lina Sarkis 1 , Lisa Sturm 1 , Vera Britz 1 , Andreas Lechner 1,3 , Anne Potzel 3,4,5 , Sonja Kunz 1 , Martin Bidlingmaier 1 , Klaus Neuhaus 6 , Adrian Gottschlich 7,8,9 , Sebastian Kobold 7,10,11 , Nicole Reisch 1 , Melanie Schirmer 2 , Martin Reincke 1 & Christian Adolf 1
1Department of Medicine IV, LMU University Hospital, LMU Munich, Munich, Germany; 2Translational Microbiome Data Integration, Technical University of Munich, Freising, Germany; 3German Center for Diabetes Research (DZD), Neuherberg, Germany; 4Physicians Association for Nutrition e.V, Munich, Germany; 5CCG Type 2 Diabetes, Helmholtz Zentrum München, Munich, Germany; 6ZIEL - Institute for Food & Health, Technical University of Munich, Freising, Germany; 7Division of Clinical Pharmacology, LMU University Hospital, LMU Munich, Munich, Germany; 8Bavarian Cancer Research Center (BZKF), Munich, Germany; 9Department of Medicine III, University Hospital, LMU Munich, Munich, Germany; 10German Cancer Consortium (DKTK), partner site Munich, a partnership between DKFZ and University Hospital LMU, Munich, Germany; 11Einheit für Klinische Pharmakologie (EKLiP), Helmholtz Munich, Research Center for Environmental Health (HMGU), Neuherberg, Germany
Background: It is known that excessive dietary sodium intake increases cardiovascular risks and contributes to elevated blood pressure. Additionally, a high-salt diet is linked to unfavorable inflammatory immune responses. Notably, individuals with primary aldosteronism (PA) commonly exhibit elevated sodium intake. Despite treatment with mineralocorticoid receptor antagonists (MRA), PA patients do not experience a complete reduction in excess cardiovascular risk compared to hypertensive controls. Hence, this study aims to explore the impact of moderately reducing sodium intake on the gut microbiome composition and immunophenotype in PA patients.
Methods: Conducted as a prospective two-stage clinical trial, we compared 15 PA patients before start of MRA therapy with appropriately matched healthy controls (cohort A). Furthermore, in 31 PA patients on stable MRA treatment for at least three months, we evaluated standardized blood pressure measurements, performed laboratory tests, analyzed peripheral blood mononuclear cells using flow cytometry and collected stool samples for microbiome analysis before and after a three-months intentional reduction in dietary sodium (cohort B).
Results: Our findings unveiled alterations in the immunophenotype of PA patients following a median sodium reduction of 3.7 g/d. In cohort A, we could observe an upregulation of regulatory T cells in patients with PA compared to normotensive controls (P=0.0072) as well as a downregulation of Tc2 cells. Abundance of Bacteroides uniformis was higher in the subgroup of patients with PA compared to the matched control cohort (P=0.0123), while abundance of Lactobacillus species was reduced compared to controls (P=0.0052). Sodium reduction in cohort B led to a decrease in the percentage of pro-inflammatory and pro-hypertensive Tc17 cells (P=0.0008) with major effects in male PA patients (P=0.0012) and in Tc17/Treg ratio (P=0.0007). While sodium reduction did not lead to changes in microbiota abundance, abundance of Bacteroides uniformis was higher in female compared to male PA patients throughout the study. Abundance of Bacteroides uniformis showed a trend to decrease upon MRA treatment and sodium reduction.
Conclusion: Our study suggests a less pronounced inflammatory phenotype in patients with PA treated with moderate dietary sodium restriction. This observed immune modulation could potentially contribute to effective blood pressure reduction and positively impact cardiovascular risk. Recognizing the interplay between sodium intake, immune responses, and cardiovascular health underscores the potential therapeutic implications of dietary interventions in the management of PA and associated cardiovascular risks.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more