Endocrine Abstracts

Objective: Anaplastic Thyroid Cancer (ATC) represents a formidable challenge in oncology due to its aggressive nature and high metastatic potential. This study delves into the quantitative assessment of microRNAs (miRNAs), specifically miRNA-149-5p, miRNA-548c-3p, and miRNA-3619-3p, to elucidate their roles in ATC progression and metastasis. Employing the robust technique of quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR), we meticulously examine the expression profiles of these miRNAs in ATC tissues, juxtaposed against adjacent normal thyroid tissues. Our findings reveal distinct and quantifiable alterations in the expression levels of miRNA-149-5p, miRNA-548c-3p, and miRNA-3619-3p in ATC, providing valuable insights into their potential as diagnostic and prognostic markers. Furthermore, the study employs RT-PCR to quantitatively assess the impact of these miRNAs on key cellular processes integral to cancer progression, such as proliferation, invasion, and metastasis.

Material and Methods: miRNA-149-5p, miRNA-548c-3p, miRNA-3619-3p expression was analyzed in ATC tissue and whole blood by Real-Time Quantitative Polymerase Chain Reaction.

Results: The investigation extends to delineating the regulatory influence of the selected miRNAs on target genes associated with ATC pathogenesis. Through quantitative analyses, we unravel the intricate molecular mechanisms underlying the suppressive effects of miRNA-149-5p on cell proliferation, as well as the regulatory roles of miRNA-548c-3p and miRNA-3619-3p in modulating cellular migration and invasion. The quantitative RT-PCR data presented herein not only contribute to a comprehensive understanding of miRNA involvement in ATC but also lay the foundation for potential therapeutic interventions. The precise quantification of miRNA expression provides a basis for developing targeted therapeutic strategies aimed at modulating these molecular regulators, thereby offering new avenues for personalized treatment approaches in the pursuit of mitigating ATC aggressiveness.

Conclusion: This study, leveraging the quantitative power of RT-PCR, advances our understanding of miRNA-mediated mechanisms in ATC, presenting a promising framework for future research and therapeutic exploration in the field of precision medicine.

Keywords: Anaplastic Thyroid Cancer (ATC), miRNAs (149-5p, 548c-3p, 3619-3p), Cancer biomarkers (early diagnostic), Therapeutic targets, Prognostic factors, Epithelial-mesenchymal transition (EMT).

Disclosure of interest: None declared