ECE2024 Poster Presentations Pituitary and Neuroendocrinology (120 abstracts)
Soluble alpha klotho concentrations are resistant to short-term stimulation and suppression of growth hormone
Junia Ribeiro de Oliveira Longo Schweizer 1 , Katharina Schilbach 1 , Olivia Tausendfreund 1 , Michael Haenelt 1 , Anica Gagliardo 1 , Jochen Schopohl 1 , Michael Drey 1 & Martin Bidlingmaier 1
1Ludwig Maximilians University, LMU Klinikum; Med. Klinik und Poliklinik IV, München
Background: Serum soluble alpha klotho (sαKL) is a circulating protein which had been shown to be very high in active acromegaly, and significantly reduced after disease control. Therefore, sαKL has been suggested as a new biomarker for growth hormone (GH) excess. In this study, we aimed to evaluate the impact of short-term stimulation and suppression of GH and acute modulation of glucose metabolism on sαKL concentrations.
Methods: Serum sαKL was measured by ELISA (IBL, Hamburg, Germany). Samples were collected during GHRH-Arginine test (0, 30, 60, 90 and 120 min.) in geriatric patients without GH deficiency (n=9, 72-94 years, 5 males) and in a cohort of patients with proven GH deficiency (GHD, n=10, 22-71years, 5 males). We also evaluated sαKL in healthy participants (n=22, aged 22-52 year, 8 males) during oral glucose tolerance test (OGTT: 0,30,60,90,120 and 180 min.).
Results: As expected, GH concentrations (mean±SD, ng/dl) significantly increased during GHRH-Arginine test in participants without GHD (baseline: 0.78±0.48 vs peakGH: 14.7±7.8, P<0.05) but not in patients with GHD (baseline: 0.36±0.5 vs peakGH: 1.26±1.1, p>0.05). Neither in aged patients without GHD (baseline sαKL: 668±174 vs 120minsαKL: 654±168, p>0.05) nor in patients with GHD (baseline: 831±221 vs 120minsαKL:827±271, p>0.05) sαKL concentrations (mean±SD, pg/ml) varied significantly during GHRH-Arginine test. During OGTT, GH concentrations decreased, and glucose and insulin increased, but sαKL concentrations did not change significantly (p>0.05 for all comparisons).
Conclusion: In contrast to chronic GH excess in acromegaly, sαKL concentrations are not significantly affected by short-term stimulation and suppression of GH. Furthermore, acute elevation of glucose and insulin do not significantly impact sαKL concentrations in healthy subjects.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more