ECE2024 Eposter Presentations Thyroid (198 abstracts)
Exploring thyroid effects and gender-specific responses to pseudomonas aeruginosa mannose-sensitive-hemagglutinin (PA-MSHA): a rat model study
Katarina Baralić 1 , Dragica Bozic 1 , Đurđica Marić 1 , Jovana Živanović 1 , Katarina Živančević 1 , Evica Antonijević Miljaković 1 , Aleksandra Buha 1 , Marijana Ćurčić 1 , Zorica Bulat 1 , Biljana Antonijević 1 & Danijela Đukić-Ćosić 1
1Department of Toxicology ‘Akademik Danilo Soldatović’, University of Belgrade — Faculty of Pharmacy, Belgrade, Serbia
Pseudomonas aeruginosa mannose-sensitive-hemagglutinin (PA-MSHA) is a bacteria-mediated cancer therapy showing promise in inhibiting cancer growth by impeding tumor cells and inducing natural cell death. Clinical studies indicate its ability to enhance chemotherapy efficacy and stimulate the immune system against cancer, presenting manageable side effects such as fever and skin irritation. Nevertheless, the safety profile of PA-MSHA requires further exploration, emphasizing the necessity for additional investigation. Hence, the aim of the current study was to investigate the thyroid effects of PA-MSHA in a rat model and elucidate gender-specific differences. Animals were subjected to a 28-day exposure to three increasing doses of PA-MSHA (PA-MSHA 1, PA-MSHA 2, and PA-MSHA 3: 0.09; 0, 18 and 0, 36×109, respectively), administered intraperitoneally once a week on the 1st, 7th, 14th, and 21st days. Following the conclusion of the study period, blood samples were obtained through cardiac puncture, and serum was isolated for subsequent hormone analysis. The levels of triiodothyronine (T3), thyroxine (T4), free triiodothyronine (fT3), and free thyroxine (fT4) were quantified, and the T3/T4 ratio was calculated. The results revealed gender-specific hormonal responses to varying doses of PA-MSHA. In females, both PA-MSHA 1 and PA-MSHA 3 significantly elevated T3 levels, with PA-MSHA 3 exhibiting a more pronounced effect, suggesting a potential dose-dependent response. Additionally, both doses induced comparable increases in fT3, indicating a potential threshold effect or saturation point in hormonal response. In males, PA-MSHA 2 led to a decrease in fT3 levels, suggesting a specific response to the intermediate dose, while PA-MSHA 3 showed a dose-dependent increase in fT4 levels, akin to the observed effect on T3 levels in females. However, both in males and in females, the absence of a statistically significant difference in the T3/T4 ratio suggests that the relative balance between T3 and T4 remained relatively constant across the experimental groups and the control, indicating that the overall thyroid hormonal equilibrium may have been maintained. However, these findings collectively suggest differential hormonal responses to varying doses of PA-MSHA in female and male animals, emphasizing the importance of gender-specific considerations in the development and interpretation of therapeutic interventions involving PA-MSHA.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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