Endocrine Abstracts

Introduction: To date, there was evidence for an association between lower serum 25(OH)D levels and higher prevalence of obesity in individuals with type 2 diabetes mellitus (DM). Vitamin D status is inversely associated with the prevalence of obesity in patients with insulin resistance syndrome. Actually, no study that has been specifically performed to investigate the association between vitamin D levels and all the parameters of body composition in obese males with type 2 DM in Ukraine.

Aim of the study: was detect 25-hydroxyvitamin D (25(OH)D) level in obese male with type 2 DM depending on fat mass (FM).

Materials and methods: A total of 31 males with type 2 diabetes have been examined and divided into II groups according to body mass index (BMI): I group (n=15) overweight subjects (BMI 26.0-29.9 kg/m²) and II group (n=16) male with obesity (BMI ≥ 30.0 kg/m²). The participants were on average 54.6±6.9 years, type 2 diabetes duration of 12.2±7.6 years, mean value of glycated hemoglobin (HbA₁C) – 7.7±1,1%. BMI was calculated by a ratio of body weight (in kilograms) and square of height (in meters), expressed in kg/m². Body composition parameters (FM, fat free mass (FFM), body cell mass (BCM), total body water (TBW)) were measured by electrical bioimpedance analysis. Serum level of 25(OH)D was detected by electrochemiluminescent method (Roche Diagnostics, Germany) and cobas test system.

Results: Analysis of 25(OH)D level depending on BMI showed revealed the highest level 25(OH)D in male with overweight (28.5±7.3 ng/ml), while the lowest level were in male group II (14.8 ±8.2 ng/ml). Male with type 2 DM of II group have a higher FM level than patients of I group (35.8±6.8 kg vs 31.2±1.9; P<0.001). Univariate analysis showed significant differences in FFM(II group: 68.5±9.7 kg vs I group: 50.2±1.8 kg, P<0.01); BCM (II group: 41.1±5.5 kg vs I group: 33.2±1.1 kg, P<0.01); TBW (group: 52.7±6.3 kg vs I group: 40.4±5.9 kg, P<0.01) between II and I groups. The highest correlation between the 25(OH)D levels and FM was observed in patients with obesity (r= -0.26; P<0.01), and significantly and negatively correlated with BMI (P<0.001) and HbA₁C (P<0.01).

Conclusion: This study confirms that obesity in males with type 2 DM is associated with vitamin D deficiency and it shows detected significant influence FM on the progression lower serum vitamin D.