Endocrine Abstracts

The present study was conducted to investigate the signaling pathway involved in betanin against noise and scrotal hyperthermia and to clarify whether it affects oxidative stress, apoptosis and inflammation in testis of rats. Scrotal hyperthermia was induced by heat exposure of rat testicular for 43ºC for 15 min (3 times per weeks for 14 days). Noise induction was done following exposure of rats with 100 decibel noise level for 14 day and 8 h daily similar to real condition. Betanin at the sub-effective dose of 15 mg/kg/day was administrated by gavage for 4 weeks (5 times in week) to male rats. The animals were sacrificed and testis were dissected and collected in freezer (-80°C). Then, the oxidative stress biomarkers, nitric oxide (NO) level, apoptosis, and inflammatory cytokines levels were studied by the real time polymerase chain reaction (RT-PCR). Our data revealed that noise and scrotal hyperthermia as pollutant caused to testicular toxicity in wistar rats via induction of oxidative damage, apoptosis and inflammatory mediators. In addition, betanin suppressed oxidative stress biomarkers apoptosis induction and caused to significantly decline in TNF-α and IL-6 gene expression level. betanin exhibited protective effects in testis following scrotal hyperthermia and noise induction through multiple pathways. Our results suggest that betanin may attenuate noise and scrotal hyperthermia induced testicular toxicity via inhibition of oxidative stress, apoptosis and inflammatory gene expression level.

Keywords: Noise; Scrotal hyperthermia; Betanin; Oxidative stress; Apoptosis; Inflammation; gene expression