EYES2023 ESE Young Endocrinologists and Scientists (EYES) 2023 Oral communication 7: Diabetes, Obesity and Metabolism (7 abstracts)
Amino acids and biogenic amines circadian fluctuation in health and in hypercortisolism states
Greta Galante 1 , Lorenzo Tucci 2 , Ilaria Improta 3 , Valentina Bissi 4 , Alessandro Perrone 4 , Kimberly Coscia 4 , Giacomo Colombin 4 , Ilaria Di Cintio 4 , Valentina Vicennati 3 , Uberto Pagotto 5 , Guido Di Dalmazi 6 & Flaminia Fanelli 7
1Endocrinology and Prevention and Care of Diabetes Unit, Center for Applied Biomedical Research, Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy, Irccs Azienda Ospedaliero-Universitaria DI Bologna, Bologna, Italy., Department of Medical and Surgical Sciences, Bologna, Italy; 2Alma Mater Studiorum University of Bologna, Irccs Sant’orsola Polyclinic Endocrinology Unit, Medical and Surgical Sciences, Bologna, Italy; 3Alma Mater Studiorum University of Bologna, Irccs Sant’orsola Polyclinic Endocrinology Unit, Italy; 4Endocrinology and Prevention and Care of Diabetes Unit, Center for Applied Biomedical Research, Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy, Irccs Azienda Ospedaliero-Universitaria DI Bologna, Bologna, Italy; 5St. Orsola General Hospital, Internal Medicin/Endocr Unit, Bologna, Italy; 6Division of Endocrinology, Dept. of Medical and Surgical Sciences, Division of Endocrinology, Department of Medical and Surgical Sciences, Alma Mater University of Bologna, S. Orsola-Malpighi Hospital, Bologna, Italy, Bologna, Italy; 7Center for Applied Biomedical Research, Dept. Of Medical and Surgical Sciences, Alma Mater Studiorum – Bologna University, Bologna, Italy, Department of Medical and Surgical Sciences (Dimec), Alma Mater Studiorum, University of Bologna, Italy.
Background: Chronic hypercortisolism exhibits irregular circadian rhythm and deranged protein metabolism. Altered amino acid(AA) and biogenic amine(BA) circulating levels were found in patients with hypercortisolism. However, there is limited information about the physiologic circadian fluctuation of AA and BA levels, and it is not known whether this is affected by states of hypercortisolism.
Aim: To characterize levels and daily fluctuations of AA and BA in convenient dried blood spots (DBS) from finger-prick in healthy subjects(HS) and in patients affected by autonomous cortisol secretion(ACS) or Cushing syndrome(CS).
Methods: HS (n=9), ACS (n=6) and CS (n=5) patients underwent a 7-days standardized isocaloric Mediterranean diet. On the 7th day, subjects collected DBS 30 min before and 2 h after breakfast, lunch and dinner and at bedtime. 21 AA and 21 BA were measured in DBS by LC–MS/MS.
Inizio modulo
Results: Compared to HS, ACS patients had lower His(P=0.026), while CS patients had lower Asn(P=0.030) and higher spermidine(P=0.003). CS also had higher spermine(P=0.028) and t4-OH-proline(P=0.032) when compared to ACS patients. A daily rhythm was detected for 11 AA in HS (e.g.: Met, Leu, Ile and Arg; P:0.001–0.006), mostly with levels higher at awakening and bedtime, and lower in the morning. Of these, some maintained their rhythm also in ACS(e.g.: Ile and Leu; both P:0.001) and CS patients(Leu; P:0.006). Fluctuation of other compounds were found in ACS (e.g.: Gly and Ser; P:<0.001) and in CS (ADMA, creatinine, spermine; P:0.003–0.018).
Conclusions: In health condition, a panel of AA displayed a diurnal fluctuation consistent with physiologic night protein catabolic processes. Daily fluctuations were revealed in ACS for a different panel of AA, and were almost all lost in CS. In addition, fluctuations in some BA were specifically detected in ACS and CS. Our data highlighted deranged histidine metabolism in ACS, and increased production of spermidine and spermine in CS.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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