ETA2023 Poster Presentations Thyroid Hormone Transport & Metabolism Basic (8 abstracts)
Types 2 and 3 deiodinase are induced in blood cells of critical ill patients: Implications to nonthyroidal illness syndrome pathophysiology
Thaliane Oliveira 1 , Milla Dheher 1 , Andre Braun 2 , Josi Vidart 2 , Rafael Marschner 1 , Bruno Kotzian 3 , Bruna Ulian 3 & Simone Wajner 4
1Universidade Federal Do Rio Grande Do Sul, Brazil; 2Universidade Federal Do Rio Grande Do Sul, Hospital de Clinicas de Porto Alegre, Brazil; 3Hospital de Clinicas de Porto Alegre, Brazil; 4Ufrgs, Hcpa, Internal Medicine, Porto Alegre, Brazil
Dysregulation of types 2 and 3 deiodinases (D2 and D3) are present and profoundly alters the metabolism of thyroid hormones in the nonthyroidal iIllness syndrome (NTIS). The activation of this enzyme in peripheral blood cells can be one of the factors that alters the progression of this syndrome. However, the effect of oxidative stress on D3 expression in these cells from ill patients is unknown.ObjectiveEvaluate the presence and variations of D3 expression in the granulocytes and lymphocytes cells of intensive care patients in ICU due to any disease.
Methods: Forty patients admitted to ICU due to any cause had their blood collected at admission and after seven days. Cells from whole blood were separated by different cell gradients and by flow cytometry. The total carbonyl content and GSH were used as a parameter of intracellular redox balance. D2 and D3 expression and locations was determined with in situ hybridization RNAscope.
Results: T3 levels were divided into three categories (T3<35ng/dl, 35-60 ngd/l and >60ng/dL). Media was 31±1.6, 47±7.6 and 63.12±3.14 ng/dL respectively. The formation of carbonyls, a marker of oxidative damage to proteins, was increased in both cell types (all P < 0.0001) in all patients, independently from admission cause. GSH levels were also diminished (P < 0.001). DIO2 and DIO3 expression were increased in activated granulocytes (by 5-fold each, P = 0.002) at admission. We observed a significant difference on D2 and D3 expression among dose patients from the groups of T3<35 and T5 35-60ng/dL when compared to the expression observed in those patients with T3>60 ng/dL RNAscope confirmed the presence of these enzymes in both granulocytes and mononuclear cells.ConclusionCritical disease induces D2 and D3 in defense cells, regardless the type of disease, contributing to diminishing circulating T3 levels.
Volume 92
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Endocrine Abstracts
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