ETA2023 Poster Presentations Miscellaneous 2 (9 abstracts)
Evaluation of advanced end glycation products (AGES), their solube receptors (SRAGE) and the components of oxidative stress in patients with hashimoto’s thyroiditis on levothyroxine substitution
Sara Csiha 1 , Istvan Molnar 2 , Sandor Halmi 3 , David Hutkai 4 , Hajnalka Lorincz 5 , Sandor Somodi 6 , Monika Katko 3 , Mariann Harangi 7 , Gyorgy Paragh 5 , Endre V. Nagy 3 , Miklos Bodor 1 & Eszter Berta 8
1Division of Endocrinology, Department of Medicine, Faculty of Medicine, University of Debrecen, Department of Clinical Basics, Faculty of Pharmacy, University of Debrecen, Hungary; 2Department of Clinical Basics, Faculty of Pharmacy, University of Debrecen, Division of Metabolism, Department of Medicine, Faculty of Medicine, University of Debrecen, Hungary; 3Division of Endocrinology, Department of Medicine, Faculty of Medicine, University of Debrecen, Hungary; 4Division of Nephrology, Department of Medicine, Faculty of Medicine, University of Debrecen, Hungary; 5Division of Metabolism, Department of Medicine, Faculty of Medicine, University of Debrecen, Hungary; 6Division of Metabolism, Department of Medicine, Faculty of Medicine, University of Debrecen, Department of Emergency Medicine, Faculty of Medicine, University of Debrecen, Hungary; 7Division of Metabolism, Department of Medicine, Faculty of Medicine, University of Debrecen, Institute of Health Studies, Faculty of Health Sciences, University of Debrecen, Hungary; 8Department of Endocrinology, Division of Metabolism, Department of Medicine, Faculty of Medicine, University of Debrecen, Department of Clinical Pharmacology, Debrecen, Hungary
Background: Advanced glycation end products (AGEs) are heterogenous group of irreversible chemical moieties originated from non-enzymatic glycation and oxidation of proteins, nucleic acids, and lipids. The engagement of AGEs with their chief cellular receptor (RAGE) activates signaling pathways contributing to the progression of chronic diseases like autoimmune thyroiditis, type 2 diabetes mellitus and its complications. Soluble RAGE (sRAGE) prevents AGE-RAGE interaction in a competitive manner. Paraoxonase 1 (PON1) is an antioxidant enzyme with reduced activity in several autoimmune diseases.
Subjects and Methods: We investigated the association between serum AGE, sRAGE and paraoxonase-1, aryl esterase activity (ARE); and thyroid function in 73 Hashimoto’s thyroiditis patients (HT) on levothyroxine (LT4) substitution, and in 83 age, BMI and gender-matched healthy controls. The serum AGEs levels were determined by autofluorescence on a multi-mode microplate reader, and the serum sRAGE levels by ELISA method. Serum PON1 paraoxonase and aryl esterase activity were detected spectrophotometrically.
Results: Mean AGE level was lower (10.71 vs 11.45 AU/µg protein; P = 0.046), while mean sRAGE level was higher (923 vs 755 pg/mL; P < 0.0005) in the serum of HT patients than the controls. AGE correlated with age, while sRAGE correlated negatively with BMI in both groups. We found negative correlation between AGE and fT3 levels (r=-0.32; P = 0.006) and sRAGE and TSH levels (r=-0.27; P = 0.022) in HT patients, while we failed to find association between AGE, sRAGE and parameters of thyroid function in the control group. Among patients AGE and RAGE levels showed no correlation with paraoxonase or aryl esterase activity of PON1.
Conclusion: According to our results in HT patients lower TSH and higher fT3 levels within the reference range is accompanied by a favourable AGE/RAGE balance. The levels of AGE and RAGE seem to be not associated with PON1 activity in LT4-treated HT patients. Further investigations are needed to confirm these results.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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