Endocrine Abstracts

Thyroid papillary carcinoma (PTC) is the most common endocrine tumor, Hashimoto’s thyroiditis (HT) is considered to be closely related to PTC pathogenesis, but their relationship still needs verification and exploration. Aim of the study. To analyze the differences and similarities of the metabolic mechanism of thyroid papillary carcinoma in normal and HT background, and to explore the relationship between HT and PTC. The Ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF/MS) technology was used to analyze 61 PTC patient tissues (31 HT background and 30 normal tissue (NC) background). Potential biomarkers were screened from principal component analysis (PCA) to orthogonal partial least square (OPLS) discriminant analysis and further evaluated by ROC analysis. The HMDB was searched to identify potential differential metabolites and the final metabolic pathway analysis was performed by MetaboAnalyst 5.0. The HT and NC group had common metabolic pathways, such as taurine and hypo-taurine metabolism, arginine biosynthesis, alanine, aspartate and glutamate metabolism, arginine and proline metabolism and D-glutamine and D-glutamate metabolism. Furthermore, arginine, glutamic acid, cysteine, citric acid, malic acid, uracil and taurine showed statistical significance in PTC diagnosis (the area under the curve values of HT group and NC group were 0.867 and 0.973 respectively). The HT group had specific metabolic pathways, including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism. The metabolic profiles of the NC and HT groups had important similarities and differences in thyroid cancer. The correlation of PTC with HT may be related to aminoacyl-tRNA biosynthesis, serine and threonine metabolism.

Keywords: Metabolomics; Hashimoto’s thyroiditis (HT); Papillary thyroid cancer (PTC); Metabolism pathway; Biomarkers