Endocrine Abstracts

Introduction: The presence of diabetes-associated antibodies is an indicator of β-cell destruction. Our goal was to find out the prevalence of autoantibodies associated with the new onset of type 1 diabetes mellitus (DM1) without family history.

Subjects and Methods: 22 patients (10 women and 12 men) with newly diagnosed DM1 no family history were examined. Average age of patients was 31.7±7.3 y.o., experience of DM1 was 2.3±0.8 months. All the patients underwent the determination of autoAbs, such as glutamic acid decarboxylase (GAD-Ab), islet antigen-2 (IA2-Ab), and islet cell cytoplasmic (ICA-Ab), zinc transporter 8 (ZnT8-Ab). The determination of the level of antibodies was carried out by the method of quantitative enzyme immunoassay using the analyzer Freedom Evo (Tecan).

Results: GAD-Ab were found in 90% of the examinees. IA2-Ab were observed in 36%. ZnT8-Ab were detected in 14 patients (63.6%). Auto-Antibodies were more frequently observed in combination than alone. 13 patients (59%) had ZnT8-Ab and GAD-Ab. IA2-Ab, GAD-Ab and ZnT8-Ab were detected in 5 patients (22.7%). ICA-Ab were not detected.

Conclusion: The antibodies that are most frequently observed in newly diagnosed DM1 are GAD-Ab, IA2-Ab and ZnT8-Ab. Along with GAD-Ab antibodies, it is possible to determine ZnT8-Ab and IA2-Ab antibodies, since ZnT8-Ab and IA2-Ab are detected in 63.6% and 36% of patients, respectively. We have not identified ICA-Ab, which confirm the usefulness of this investigation.