Endocrine Abstracts

Introduction: Diabetes Mellitus (DM) is one of the Global pandemics despite to new knowledges and methods of prevention and therapy intensively increased among children, adolescents and adults. Many studies shown the role of the gastrointestinal microbiocenosis in the development and clinical future of DM. Gut microbiota produce numerous bioactive molecules, which can act locally and secreted into bloodstream. The aim of our study were evaluation of gut microbiota in children and adolescent with DM1.

Material and Methods: In 54 patients with MD1, 21 boys and 33 girls in age from 6 to 18 years old blood fasting glycemia, HbA1c level were measured; gut microbiome status were estimated by number of E.coli, lactobacillus, staphylococci, mycobacterium in feces using PCR.

Results: Fasting glycemia level in patients were varied between 5.9 and 13.1 and HbA1c level were 12.9±0.3%. Results of studying the violation of gut microbiocenosis in children with type 1 diabetes showed decrease in the total number of anaerobes and active reproduction of aerobes, including opportunistic pathogens. Thus, they had a deficiency in the total number of anaerobes, bifidobacteria and lactobacilli were found in 100% of cases (P < 0.01). At the same time, the content of almost all aerobes significantly increases, especially lactose-negative Escherichia coli, fungi of the genus Candida and Proteus - 6.86±0.165; 6.21±0.28; 4.8±0.32 lg CFU/g, respectively. The most characteristic decrease in the number of lactose-positive Escherichia coli to 6.75±0.59 lg CFU/g against the basic increase in the content of lactose-negative Escherichia coli, enterobacteria, staphylococci, fungi of the genus Candida, especially Proteus (P<0.001). Dysbiotic changes in most cases were accompanied by the isolation of bacteria of the opportunistic pathogenic group. Intestinal microflora had differences depending on the duration of the disease and dysbiotic changes were more pronounced in those with disease duration more than 6 years, than those with less than 3 years.

Conclusion: Gut microbiocenosis were changed in patients with DM1 where disbiotic changes shown in decreasing of anaerobes and increasing of aerobes, including opportunistic pathogens. Gut microbiome state may reflected on glycemia level and depends from duration of diabetes.