ECE2023 Poster Presentations Reproductive and Developmental Endocrinology (108 abstracts)
Correlation between both serum and seminal fluid vitamin D levels and vitamin D receptor CDX polymorphism in male infertility
Suzana Vladoiu 1 , Anca Botezatu 2 , Andrei Muresan 3 , Adrian Albulescu 2 , Oana-Monica Popa 1 , Fudulu Alina 2 , Ples Adriana 2 , Cristina Stancu 1 , Iancu Iulia Virginia 2 , Corin Badiu 4,5 & Dinu Draganescu Daniela 1
1National Institute of Endocrinology, Molecular, Cellular and Structural Research Laboratory, Bucharest, Romania; 2‘Stefan S. Nicolau’ Institute of Virology, Department of Molecular Virology; 3National Institute of Endocrinology, Molecular, Cellular and Structural Research Laboratory; 4‘C. Davila’ University of Medicine and Pharmacy, Endocrinology, Bucharest, Romania; 5National Institute of Endocrinology, Thyroid Related Disorders, Bucharest, Romania
Vitamin D (VD) now considered a steroid hormone, has been correlated with male reproductive disorders. The activity of VD is mediated through its receptor (VDR), distributed in various tissues including reproductive tissues. VDR polymorphism located in the promoter region of the gene, rs17883968 (Cdx2) G → A associated with transcriptional activity. The aim of this study was to assess both seminal and serum VD levels in a group of infertile patients (n=87, median: 34 years, range 20–55 years) vs control (n=24, median: 33 years, range 20–45 years) to establish the relation with Cdx2 VDR polymorphism. Subjects presenting known causes of infertility were excluded from the study. The patients were divided in three groups according to sperm count analysis: oligoasthenospermia, oligoterato-asthenospermia and azoospermia group. In this purpose, freshly collected blood was drawn from the subjects. Seminal fluid was harvested after 3–5 days of sexual abstinence. 25(OH) Vit. D serum and seminal levels were assayed using the electrochemiluminescence method according to the manufacturer’s recommendations (Roche Diagnostics, Basel, Switzerland) with the RocheCobas E601 autoanalyzer. CDX polymorphism was evaluated through specific VDR gene PCR followed by digestion with the restriction enzyme Bpu10I (New England Biolabs). The VD serum levels was found to be low in the subjects infertile compared to the control group, and the analysis of the three groups of infertile vs. control shows differences Statistically significant for oligoasthenospermia (P=0.0109), oligoteratoasthenospermia (P=0.0420). No notable differences were observed in the seminal fluid. However, the VD levels were higher in seminal fluid than serum in all groups and a strong statistical significance was observed, suggesting the ability of reproductive tract to synthesize VD locally. VD levels were found to be significantly decreased in subjects with CDX- AA genotype especially in azoospermia (P=0.0028) and oligoasthenoteratospermia (P=0.0198). This study demonstrates a direct and positive relationship between serum VD level and overall semen quality. The results provide new insights into the involvement of the Cdx-2 polymorphism and VD deficiency in male infertility, providing genetic evidence for the role of the VDR in the development of male infertility. Taking into account the results, we highlight the important role of genetic changes at the VDR level associated with VD deficiency in male infertility.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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