Endocrine Abstracts

Background: Obesity is the major health problem in both developed and developing countries, due to increasing its rate there is a new strategy to prevent obesity by time restricted feeding (TRF). The aim of our study is the effect of TRF intervention on diet induced obese rats and their association with circadian gene expression.

Method: Total 15 Wistar rats were included in our study and divided into two groups. Control group and High Fat diet (HFD) group which consist of six rats and nine rats respectively. HFD group was fed fatty diet for two months to developed obesity. These rats were shifted to TRF with HFD for three months, after which they were again put back on ad lib (24 hr feeding). These rats were sacrificed and samples collected. Body weight was measured monthly, with blood glucose, Insulin, melatonin lipid profile and RNA expression estimated after sacrifice.

Results: The body weight and blood glucose level of HFD group were significantly increased as compared to control rats (P=0.0263) and (P=0.0089) respectively. The level of insulin, melatonin (P=0.006) and HDL were reduced in rats fed with HFD whereas total cholesterol, TG and LDL were increased. TRF intervention reduced body weight, blood glucose level, TG, LDL Per1 expression and elevated the level of insulin, melatonin, total cholesterol, HDL and Bmal1 expression. TRF in HFD induced obesity showed its legacy effect when put on ad lib.

Conclusions: TRF is a potential behavioural intervention which is easily adaptable in lifestyle modification. TRF intervention can prevent and treat obesity and their associated disorders.