ECE2023 Poster Presentations Diabetes, Obesity, Metabolism and Nutrition (159 abstracts)
The concentration of Polycyclic Aromatic Hydrocarbons (PAHs) in human serum and adipose tissues and In Vitro stimulatory effect of naphthalene in adipogenesis in 3T3-L1 cells
Ewa Mlyczyńska 1,2 , Alice Bongrani 3,4,5 , Christelle Rame 3,4,5 , Małgorzata Węgiel 6 , Anna Maślanka 6 , Piotr Major 7 , Piotr Zarzycki 7 , Pierre-Henri Ducluzeau 3,4,5,8 , Arnaud De Luca 8,9 , Celine Bourbao-Tournois 10 , Pascal Froment 3,4,5 , Agnieszka Rak 1 & Joëlle Dupont 3,4,5
1Jagiellonian University, Institute of Zoology and Biomedical Research, Laboratory of Physiology and Toxicology of Reproduction, Krakow, Poland; 2Jagiellonian University, Doctoral School of Exact and Natural Sciences, Krakow, Poland; 3INRAE, UMR 85 Physiologie de la Reproduction et des Comportements, Nouzilly, France; 4CNRS, UMR 7247 Physiologie de la Reproduction et des Comportements, Nouzilly, France; 5Université de Tours, Department of Animal Physiology, Tours, France; 6Cracow University of Technology, Faculty of Chemical Engineering and Technology, Krakow, Poland; 7Jagiellonian University Medical College, 2nd Department of General Surgery, Krakow, Poland; 8CHRU of Tours, Department of Endocrinology-Diabetology and Nutrition, Tours, France; 9University of Tours, INSERM, Nutrition, Growth and Cancer (N2C) UMR 1069, Tours, France; 10University Hospital of Tours, Department of Digestive Surgery, Tours, France
Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants derived from the incomplete combustion of organic materials. In urban and industrial areas, level of pollution with these compounds is high, which leads to numerous negative health effects. Some literature data indicate the involvement of PAHs in the development of obesity, however, there is no data showing the distribution patterns of PAHs in human adipose tissue (AT) and their impact on the process of adipogenesis. The aim of our study was to investigate the concentration of 16 priority PAHs in the plasma and three types of AT: mesenteric, omental, and subcutaneous in French and Polish bariatric patients (n=10 per group). Next, we analyzed correlation between concentration of the four most abundant PAHs and BMI of patients as well level of adipokines in plasma and AT. Using the mouse preadipocytes 3T3-L1 cell line we determined the dose-dependent effect of naphthalene (1-500 ng/ml) on adipocytes viability (CK-8 assay), proliferation (BrdU assay) and differentiation (Red oil staining, qRT-PCR) as well effect on its endocrine function (ELISA assay) (n=6). Statistical analysis was carried out using the Student’s t-test or one-way ANOVA to compare differences between group (P≤ 0.05) while correlations were analyzed with the Pearson correlation coefficient, two-tailed (P≤ 0.05). We observed that the concentrations of the 16 tested PAHs were higher in adipose tissue than in plasma, indicating bioaccumulation. In addition, in French patients, the concentration of naphthalene in both plasma and omental AT correlated with BMI as well as the concentration and expression of the gene for adiponectin, chemerin and resistin. We demonstrated for the first time that naphthalene stimulates viability and proliferation of 3T3-L1 cells. Moreover, it has a significant impact on the differentiation of preadipocytes by increasing lipid content in cells, upregulation of genes involved in adipogenesis, and stimulating chemerin secretion by differentiated 3T3-L1 cells. Taken together those findings, we conclude that PAHs and more particularly naphthalene could be an obesogen molecule and increase the risk of obesity. Funding: Study was supported by Région Centre Val de Loire (HAPOFERTI project number 32000496). Ewa Mlyczynska obtained funding as a part of PhD scholarship program—BGF doctorate from Campus France and Jagiellonian University programs: Excellence Initiative –Jagiellonian University.
Volume 90
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Endocrine Abstracts
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