ECE2023 Eposter Presentations Thyroid (128 abstracts)
Role and expression of miRNAs (miRNA 149_5p, miRNA-548c-3p, miRNA 3619-3p) in Papillary thyroid cancer progression and metastasis
Ramesh Bangaraiahgari 1 , Rajesh Bangaraiahgari 2 , Ramakanth Bhargav Panchangam 3 , Udaya kumar Neelagiri 4 , Srinivas Vudathala 5 , Rajkiran Reddy Banala 6 & Chakrapani Bangaraiahgari 7
1Arundathi Institute of Medical Sciences, Biochemistry, Hyderabad, India; 2Arundathi Institute of Medical Sciences, Anatomy, Hyderabad, India; 3Bhargav Endocare Hospital, Endocrine and Metabolic Surgery, Andhra pradesh, India; 4Arundathi Institute of Medical Sciences, Pediatrics, Hyderabad, India; 5Arundathi Institute of Medical Sciences, CRL, Hyderabad, India; 6Sunshine Hospital, CRL, Hyderabad, India; 7Chakri Neuro Hospital, Neurology, Nizamabad, India
Objective: Papillary thyroid carcinoma (PTC) is themalignantcancer with altered microRNA (miR) changes. miR149-5p and miR-548c-3p participates in multiple processes of tumor development, progression, tumor suppression. Whereas miR-3619-3p plays role to promote cell migration and invasion in PTC. However, the role of miR149, miR-548c-3p, and miR-3619-3p in PTC and the underlying mechanisms remain undefined. Therefore, the present study is aimed to quantify therelative expression of these 3 miR’s in tissues and whole blood of PTCpatients to understand the underlying mechanism in metastasis and reoccurrence.
Materials and Methods: miR149, miR-548c-3p, and miR-3619-3pexpression was analyzed in PTC tissue and whole blood by Real-Time quantitative Polymerase Chain Reaction.
Results: Our results indicated that miR-149-5p and miR-548c3p was downregulated and simultaneously, the miR-3619-3p expression was found to be upregulated in the tissues and whole blood of PTC patients. Moreover, downregulation ofmiR-149-5p and miR-548c-3p will help in cell viability, colony formation, cell migration, invasion capacity, upregulation of N-cadherin and vimentin genesin both PTC and whole blood samples. A negative correlation was determined betweenmiR’s (miR-149-5p and miR-548c-3p) and miR-3619-3p, hypoxia-inducible factor (HIF) 1α or vascular endothelial growth factor (VEGF) levels, indicating that miR149-5p and miR548c-3p inhibited tumor progression by suppressing the HIF1α-mediated VEGF signalling pathway.
Conclusions: Higher expression miR149-5p and miR-548c-3p could suppress PTC progression by inhibiting the HIF1α-mediated VEGF signalling pathway.
(Key Words Thyroid cancer, Papillary thyroid carcinoma (PTC), NGS = Next Generation Sequencing, Genomics; Mutation).
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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