ECE2023 Eposter Presentations Thyroid (128 abstracts)
The role of PTEN methylation level changes in papillary thyroid carcinoma diagnosis and prognosis
Mintaute Kazlauskiene 1 , Kristina Zukauskaite 2,3 , Raimonda Klimaitė 1,4 , Aistė Kondrotienė 1,4 , Dalia Daukšienė 1 , Birute Zilaitiene 1,4 , Rasa Sabaliauskaite 2,3 & Albertas Dauksa 5
1Lithuanian University of Health Sciences, Institute of Endocrinology, Lithuania; 2National Cancer Institute, Laboratory of Genetic Diagnostic, Vilnius, Lithuania; 3Vilnius University Life Sciences Centre, Institute of Biosciences, Vilnius, Lithuania; 4Hospital of Lithuanian University of Health Sciences Kaunas Clinics, Department of Endocrinology, Kaunas, Lithuania; 5Lithuanian University of Health Sciences, Institute of Digestive Research, Kaunas, Lithuania
Introduction: Papillary thyroid cancer (PTC) is the most common type of thyroid cancer (85%–90%). Today Fine needle aspiration biopsy (FNAB) is the diagnostic tool for the evaluation of thyroid nodules because of its accuracy and cost-effectiveness, but FNAB also has limitations, as it is quite challenging to take a biopsy from a small thyroid nodule moreover FNAB diagnosis is doubtful in up to 20% of cases. Therefore, non-invasive biomarkers of PTC are needed.
Aim of the study: To evaluate methylation level changes of the PTEN gene in FFPE tissue samples and peripheral blood plasma samples of PTC patients before and after surgery.
Methods: The study included 50 FFPE tissue samples from 25 patients and 68 patients plasma samples with a histologically confirmed diagnosis of PTC. Peripheral blood samples were collected before surgery and 4-6 weeks after surgery. Methylation level changes of the PTEN gene were analysed in plasma and FFPE tissue samples by quantitative methylation-sensitive polymerase chain reaction.
Results: Paired sample analysis showed statistically higher PTEN methylation levels in FFPE tumor tissue samples compared to non-cancerous tissue samples (P= 0,016). Plasma PTEN methylation levels were compared before and after PTC surgery. Significantly lower levels of PTEN methylation were found in samples collected after surgery compared to samples collected before surgery (P= 0,031).
Conclusion: PTEN methylation level changes may be a promising minimal invasive biomarker in predicting PTC prognosis and diagnosing PTC.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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