BES2022 BES 2022 Abstracts (23 abstracts)
Identification of myokines potentially involved in the improvement of glucose homeostasis after bariatric surgery
Orioli Laura 1,2 , Canouil Mickaël 3,4 , Sawadogo Kiswendsida 5 , Ning Lijiao 3,4 , Deldicque Louise 6 , Lause Pascale 1 , de Barsy Marie 2 , Froguel Philippe 3,4,7 , Loumaye Audrey 1,2 , Deswysen Yannick 8 , Navez Benoit 8 , Bonnefond Amélie 3,4 & Thissen Jean-Paul 1,2
1Pole of Endocrinology, Diabetes and Nutrition, Institute of Experimental and Clinical Research, Université Catholique de Louvain, Brussels, Belgium; 2Department of Endocrinology and Nutrition, Cliniques Universitaires Saint-Luc, Brussels, Belgium; 3Inserm U1283, CNRS UMR 8199, European Genomic Institute for Diabetes, Institut Pasteur de Lille, Lille, France; 4University of Lille, Lille University Hospital, Lille, France; 5Statistical Support Unit, King Albert II Cancer and Hematology Institute, Cliniques Universitaires Saint-Luc, Brussels, Belgium; 6Institute of NeuroScience, Université Catholique de Louvain, Louvain-La-Neuve, Belgium; 7Department of Metabolism, Digestion and Reproduction, Imperial College London, London, United Kingdom; 8Department of Oeso-gastro- duodenal and Bariatric Surgery, Cliniques Universitaires Saint-Luc, Brussels, Belgium
Purpose: Our study aims to identify myokines potentially involved in improved glucose homeostasis after bariatric surgery.
Methods: Obese patients were evaluated before and 3 months after bariatric surgery. Insulin resistance was assessed using the Homeostasis Model Assessment (HOMA) test. Muscle biopsies were taken from vastus lateralis. Genes encoding myokines involved in glucose homeostasis were identified using RNA-sequencing. Changes in myokines expression were confirmed by real-time quantitative PCR (RT-qPCR). Changes in myokines fractalkine and myostatin plasma levels were measured by ELISA. A linear regression analysis was used to predict changes in the HOMA test from changes in myokines expression or plasma levels.
Results: Insulin resistance was significantly improved (HOMA-IR, -53%, p<0.001). Up- regulated genes included CX3CL1 (encoding fractalkine, +73%, p<0.001) and BDNF (encoding Brain-Derived Neurotrophic Factor, +30%, p=0.006) while MSTN (encoding myostatin) was down-regulated (-45%, p<0.001). Plasma levels of fractalkine and myostatin were, respectively, increased (+7%, p=0.001) and decreased (-32%, p<0.001). However, changes in insulin resistance were not correlated with changes in gene expression or plasma levels of fractalkine or myostatin. In contrast, increased expression of BDNF was significantly associated with decreased insulin resistance (HOMA-IR, adjusted estimate -0.58 [-0.96; -0.19], p=0.004).
Main conclusions: Bariatric surgery is associated with both improved glucose homeostasis and changes in myokines involved in glucose homeostasis. Although gene expression and circulating levels of fractalkine and myostatin are changed by surgery, they do not correlate with changes in glucose homeostasis. In contrast, increased expression of BDNF is correlated with improved insulin resistance, suggesting its potential role in improved glucose homeostasis after bariatric surgery.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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