SFEBES2022 Poster Presentations Metabolism, Obesity and Diabetes (96 abstracts)
Effects of ethanolic extract of Allium Sativum L on the hippocampus of male Wistar rats with Streptozotocin (STZ)- induced brain insulin resistance
John Afees Olanrewaju 1 , Ayodele Roland Bejide 1 , Temitope Njideaka-Kevin 1 , Testimony Priscilla Ajibade 1 , Joseph Igbo Enya 2 , Leviticus Arietarhire 3 , Oladimeji Soremekun 1 , Stephen Adeleke 4 , Stephen Taiye Adelodun 1 , Sunday Olatunji 1 , Toluwanimi Afolabi 5 , Ayodeji Zabdiel Abijo 1 & Joshua Owolabi 1,6
1Department of Anatomy, Babcock University, Ilishan-Remo, Ogun State, Nigeria; 2Department of Anatomy, PAMO University of Medical Sciences, Port Harcourt Rivers State, Nigeria; 3Department of Anatomy, University of Ilorin, Ilorin, Nigeria; 4Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria; 5Department of Anatomy, Olabisi Onabanjo University, Ago Iwoye, Ogun State, Nigeria; 6University of Global Health Equity, Butaro, Rwanda
Alzheimer’s disease (AD) development has a direct relationship with Brain-insulin resistance. Central in the pathology of AD are beta-amyloid and tau proteins associated with cognitive decline in the hippocampus. Allium sativum L. has been shown to have a neuroprotective effect. Thirty-two male Wistar rats (80-150 g) were assigned into A-D (n=8 each), and housed in standard plastic cages at 23± 1°C. Group A received saline, groups B and C were administered 3 mg/kg of streptozotocin while C was then treated with 300 mg/kg of Allium sativim L., group D received only 300 mg/kg of Allium sativum L. Intracerebroventricular method was utilized for STZ administration; Allium sativum L. ethanolic extract administration was orogastrically; administration in all groups lasted for 21 days. Elevated plus maze and Y-maze was used to assess behaviours, after which rats were sacrificed by cervical dislocation. Following brain removal, the hippocampus was excised and processed for Cytoarchitecture (H&E, Cresyl Fast violet), Neurotransmitters (Gamma Amino Butyric Acid and Glutamate), immunohistochemical (amyloid beta protein) and RNA (Insulin receptor substrate, glycogen synthase kinase and Beta-region amyloid precursor protein cleaving enzyme 1) assays. The data obtained were analysed by ANOVA and identification of mean differences by Turkey’s posthoc test. The intervention group C showed good explorative activities, Allium sativum L, reduced significantly the expression of amyloid beta proteins as well as improved overall cytoarchitecture in the hippocampus. The increased expression of insulin, insulin receptor substrate, GSK-β3 and BACE-1 proteins in the STZ groups suggests its role in the onset of amyloidogenesis. This study reported an overall inhibition of hippocampal functions as a result of STZ as well as the neuroprotective intervention of Allium sativum L in the brain metabolic pathways whose modulatory effects should also be studied at graded doses.
Keywords: Alzheimer’s disease, brain insulin sensitivity, Allium sativum L, hippocampus
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more