ETA2022 Poster Presentations Thyroid Hormone ACTION (10 abstracts)
FAM83B is involved in thyroid cancer cell differentiation and migration
Valentina Cirello 1 , Elisa Stellaria Grassi 2 , Gabriele Pogliaghi 3 , Viola Ghiandai 4 , Laura Ermellino 5 , Marina Muzza 6 , Giacomo Gazzano 7 , Luca Persani 8 , Carla Colombo 9 & Laura Fugazzola 10
1Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano Irccs, Milan, Italy, Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy; 2University of Milan, Medical Biotechnology and Translational Medicine, Medical Biotechnology and Translational Medicine, Milan, Italy; 3Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano Irccs, Department of Pathophysiology and Transplantation, University of Milan; 4University of Milan, Laboratory of Endocrine and Metabolic Research, Istituto Auxologico Italiano Irccs, Medical Biotechnology and Translational Medicine, Milan, Italy; 5Pathology Unit, Istituto Auxologico Italiano Irccs; 6Istituto Auxologico Italiano Irccs, Division of Endocrine and Metabolic Diseases, Milan, Italy; 7Pathology Unit, Irccs Istituto Auxologico Italiano, Milan, Istituto Auxologico Italiano Irccs, University of Milan, Milan, Italy, Pathology Unit,, Milan, Italy; 8University of Milan, Irccs Istituto Auxologico Italiano, Ospedale San Luca, Milan, Italy; 9University of Milan, Department of Pathophysiology and Transplantation, Italy; 10University of Milan, Milan, Italy, Endocrine Unit, Fondazione Policlinico Irccs, Milan, Italy, Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy; Division of Endocrine and Metabolic Diseases, Istituto Auxologico Italiano Irccs, Milan, Italy
FAM83B has been recently identified as an oncogene involved in the development and progression of several malignancies, but its role in thyroid cancers (TC) is still unclear. In the present study, we examined the expression of FAM83B and its possible involvement in cell migration and differentiation, in neoplastic and normal thyroid tissues and in TC human cell lines. We found that FAM83B expression in TC varies according to tumor histotype, being significantly downregulated in more aggressive TCs and in metastatic tissues. In vitro experiments showed that FAM83B levels in cell lines recapitulate patients’ samples variations, and that its total and cytoplasmic levels decrease upon the induction of migration, together with an increase in its nuclear localization. Similar variations were detected in the primary tumor and in the metastatic tissues obtained from a patient with a follicular TC. FAM83B knock down experiments confirmed a role for FAM83B in thyroid differentiation and migration, as demonstrated by the reduction of thyroid differentiation markers PAX8 and NIS and the increase of the mesenchymal marker Vimentin. Moreover, the silencing of FAM83B significantly increased cells migration abilities, while not affecting the oncogenic RAS/MAPK/PI3K pathways. Our data indicate for the first time a role for FAM83B in TC cell differentiation and migration. Its expression is reduced in more dedifferentiated tumors and the decrease in its expression and its nuclear re-localization could favour distant migration, suggesting that FAM83B should be considered a possible diagnostic and prognostic biomarker.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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