ECE2022 Poster Presentations Diabetes, Obesity, Metabolism and Nutrition (202 abstracts)
Mediterranean Diet and 12 months weight loss result in complex serum metabolomic and fecal metaproteomic changes in paediatric obesity
Daniele Spadaccini 1 , Virginia Vita Vanella 2 , Elettra Barberis 2 , Roberta Ricotti 3 , Simonetta Bellone 3 , Elisa Bona 4 , Valentina Antoniotti 3 , Marina Caputo 1 , Valentina Mancioppi 3 , Ivana Rabbone 3 , Marcello Manfredi 2 & Flavia Prodam 1
1University of Piemonte Orientale, Department of Health Sciences, Novara, Italy; 2University of Piemonte Orientale, Department of Translational Medicine, Novara, Italy; 3University of Piemonte Orientale, SCDU of Pediatrics, Department of Health Sciences, Novara, Italy; 4University of Piemonte Orientale, Department of Sciences and Technological Innovation, Alessandria, Italy
Background: The relationship between composition and function of microbiota, obesity and nutrition has been increasingly studied and is still a challenge.
Aims: To test whether in a group of 12 pediatric patients with obesity the treatment with a structured hospital and in-home Mediterranean Diet intervention had an impact on gut microbiota composition, function, and metabolite production, including Short chain fatty acids (SCFAs).
Methods: An untargeted metaproteomics analysis through nano-LC chromatography coupled to high-resolution mass spectrometer (Triple TOF 5600+, Sciex), for the identification of phyla, and GC-MS for the analysis of SCFA and small molecules in serum and stools were carried out. All identified peptides were annotated (UniProtKB database, KEGG) and their functions were identified by UniPept software. Phylogenetic analysis was also performed. Subjects were evaluated at baseline, and after 6 and 12 months.
Results: At metaproteomic, we identified more than 250000 peptides, and 70000 proteins. We were able to identify common features between patients from the same time group. We detected changes associated with an improvement of the gut inflammatory state directly dependent on the diet. Bacterial phylogenetic and functional changes were observed in relation to the dietary intervention, BMI reduction, and adherence to the Mediterranean diet. Both after 6 and 12 months, patients lost weight and contemporarily showed a reduction of fecal SCFA (acetic, butanoic, pentanoic, and propanoic) and an increase of the Bacteirodetes/Firmicutes ratio. Acetic acid and butyric acid were also decreased in serum after 6 and 12 months. Functionally, we observed an increase in pectin catabolism. We also identified fecal sugars and amino acid changes associated to the dietary intervention.
Conclusions: This preliminary data confirm that also pediatric obese individuals losing weight reverse their microbiota, increasing their Bacteirodetes/Firmicutes ratio. At the same time, most SCFA were found to be reduced both after 6 and 12 months after the intervention. Obese individuals could have an impaired utilization of SCFA that translates into a higher lipogenic effect compared to lean individuals who might better take advantage of the anti-inflammatory role. More intervention studies should be performed in this promising area by utilizing similar innovative techniques.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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