ECE2022 Poster Presentations Pituitary and Neuroendocrinology (127 abstracts)
The immune microenvironment landscape in pituitary tumors, genes and cells
Sandra Vela Patiño 1,2 , Ma. Isabel Salazar 2 , Ilan Remba-Shapiroa 1 , Eduardo Peña-Martínez 1 , Gloria Silva-Roman 1,2 , Sergio Andoneui-Elguera 1 , Keiko Taniguchi-Ponciano 1 , Laura Bonifaz 3 , Cristina Aguilar-Flores 3 , Moises Mercado 1 & Daniel Marrero-Rodríguez 1
1Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Unidad de Investigación Medica en Enfermedades Endocrinas, Mexico; 2Escuela Nacional de Ciencias Biologicas, Laboratorio de virología e inmunovirología, Departamento de Microbiología, Mexico; 3Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Unidad de Investigación Medica en Inmunoquimica, Mexico
The tumor immune microenvironment is essential, it could influence a favorable or negative response against the tumor cells, and it has been related to therapy response and prognostic factors. Tumor infiltrating lymphocytes (TILs) plays an important role in the development, progression, and tumor control, thus presence of TILs could be related to disease-specific traits. The aim of the present work is to evaluate the expression of immune-related genes in forty-two pituitary adenomas (PA) distributed in the three major lineages, NR5A1- (Clinically non-functioning PA), TBX19- (ACTH adenoma) and POU1F1- (GH, TSH, PRL adenoma) through whole transcriptome analysis and RT-qPCR, and to identify the microenvironment immune cells infiltrating the tumor through transcriptome deconvolution and immunofluorescence. We found characteristic expression profiles of immune-related genes including interleukins and chemokines for each tumor lineage. Genes such as IL4I1, IL36A, TIRAP, IL17REL and CCL5 were upregulated in all PA whereas IL-34, IL20RA and IL2RB characterize the NR5A1-, TBX19- and POU1F1-derived tumors, respectively. Transcriptome deconvolution showed that macrophages, CD4+ T cells, CD8+ T cells, NK cells, and neutrophils could be infiltrating the PA. CD4+ and CD8+ T cells and NK cells infiltration predicted was corroborated by immunofluorescence in pituitary adenomas. In conclusion, we found characteristic immune response gene expression profiles for each pituitary tumor lineage, that explain partially, the immune response CD8+, CD4+ T cells, NK cells and macrophages infiltration. Our results suggest a crosstalk between the tumor cells and its microenvironment.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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