ECE2022 Eposter Presentations Pituitary and Neuroendocrinology (211 abstracts)
Primary cilia in Pituitary neuroendocrine tumours and their association with aggressiveness
Rebeca Martínez-Hernández 1 , Ana Serrano-Somavilla 1 , Miguel Sampedro-Nuñez 2 , Marí a Calatayud 3 , Almudena Vicente 4 , Gonzalo García-de-Casasola 5 , Ancor Sanz-Garcia 6 , Manel Puig-Domingo 7 & Monica Marazuela 1
1IIS Princesa, Universidad Autónoma de Madrid, Endocrinology, Madrid, Spain; 2Hospital La Princesa, Endocrinology, Madrid,; 3Hospital Universitario 12 de Octubre, Endocrinology, Madrid, Spain; 4Hospital Virgen de la Salud, Endocrinology, Toledo, Spain; 5Hospital Universitario Infanta Cristina, Pathology, Parla, Spain; 6IIS Princesa, Methodology Unit, Madrid, Spain; 7Germans Trias i Pujol Research Institute and Hospital, Endocrinology, Barcelona, Spain
Purpose: Although growing evidence supports the role of primary cilia (PC) in the regulation of cancer development, their possible role has not yet been studied in pituitary neuroendocrine tumours (PitNETs). The study of cilia could serve as a diagnostic tool providing new insights into the mechanisms of tumorigenesis and aggressiveness of PitNETs.
Methods: A total of 86 patients with PitNETs (28 functioning and 58 non-functioning [NF-PitNETs]) and 12 controls were evaluated by immunofluorescence and immunohistochemistry in tissue microarrays (TMA) and by western blot in PitNETs protein extracts. The frequency of ciliated cells was estimated using a cilia index score based on the length of cilia and the percentage of ciliated area in the tissue. The distribution of PC and its correlation with several clinical parameters and aggressiveness was analysed.
Results: PC were present only in scattered cells of control pituitary tissues, whereas PitNET cells showed robust staining for ciliary markers. Interestingly, the number and /or length of ciliated cells was significantly higher in non-functioning PitNETs, including null cell adenomas and gonadrotropinomas, when compared to functional tumours. Remarkably, PC were significantly increased in non-functioning-PitNETs. In addition, the presence of cilia was associated with tumour invasion, aggressiveness and recurrence in PitNETs.
Conclusions: PC are present in PitNETs, especially in non-functioning PitNETs and may represent an important contributor to aggressiveness. PC may serve as a novel diagnostic marker for predicting tumour behaviour and as a potential target for drug therapy.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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