Endocrine Abstracts

Background: Chronic kidney disease (CKD) is one of the leading causes of disability and mortality in patients with different types of diabetes mellitus (DM). However, relationships between kidney function and serum amylin levels are still controversial, especially in patients with heterogeneous types of diabetes. The objective of the study was to determine the features of the glomerular filtration rate (GFR) in patients with DM depending on the level of amylinemia.

Methods: 89 patients with DM and CKD were examined, as well as 15 representatives of the control group. The patients were divided into three groups by the types of DM (25 patients with classical type 1 diabetes mellitus (T1DM), 36 patients with latent autoimmune diabetes in adults (LADA), 28 patients with type 2 diabetes (T2DM)) and by the GFR stages (G1-21, G2-49, G3a-48, G3b-17, G4-10). GFR was determined by the CKD-EPI formula. Serum amylin levels were measured using the ELISA method.

Results: The level of amylinemia in patients with GFR stages G1 and G2 probably did not differ in comparison with the control and among themselves. In the group of patients with GFR stage G3a, the level of serum amylin was 7.7 times higher than the control, 3.7 times higher than the G1 group, but did not differ from the one in group G2. In patients with GFR of stage G3b, the above indicator was 14.7 times higher relative to the control, 7.2 times higher relative to the group of individuals with GFR of stage G1, 3.5 times higher than the G2 stage and 1.9 times higher than the G3a stage. The serum level of amylin in the group of patients with GFR stage G4 was significantly higher by 26.0 times compared to the control, 12.7 times – in the group of patients with GFR stage G1, 6.1 times – in the group of patients with GFR of stage G2, 3.4 times – in the group of patients with GFR of stage G3a and has not changed compared to the G3b group. In patients with DM positive correlations were found between the content of amylin and insulin, C-peptide, HOMA-IR index and creatinine (P<0.05), in the group of patients with T2DM - between serum amylin and GFR (P<0.05).

Conclusion: The level of amylin was increased in proportion to the stages of GFR in patients with T2DM and LADA, which indicates the role of hyperamilinemia in the development of CKD in this category of patients.