Endocrine Abstracts

Relevance: There is a search for candidate genes that contribute to the progression of atherosclerosis in patients with diabetes mellitus. It has been suggested that the FABP2 Ala54Thr polymorphism may be associated with the risk of atherosclerosis.

Objective: To evaluate the relationship between polymorphism of the intestinal fatty acid-binding protein (FABP2) gene Ala54Thr with peripheral atherosclerosis in patients with type 2 diabetes mellitus.

Materials and methods: We examined 40 patients with type 2 diabetes, who were in the endocrinology department of the Regional Clinical Hospital. N. A. Semashko in September-November 2021 The duration of diabetes mellitus is from 2 to 18 years, glycated hemoglobin is 8.1 ± 0.93%. The patients’ age ranged from 43 to 76 years old\. All patients had grade 1-2 obesity, dyslipidemia, 36 patients were diagnosed with arterial hypertension. Patients had micro and macrovascular complications of diabetes mellitus of varying severity. Glycemic indicators (HbA1c, glycemic fluctuations using FreeStyle monitoring), lipid profile, body mass index (BMI), systolic and diastolic blood pressure (SBP and DBP) were determined, ultrasound examination of the vessels of the lower extremities and the brain was performed. Molecular genetic study was carried out on the basis of the Regional Clinical Hospital named after N. A. Semashko. We saw FABP2 (rs1799883) Ala54Thr. DNA samples isolated from whole blood leukocytes using the DNA-Express-Blood-Plus reagent kit were used for analysis, followed by analysis of the isolated DNA by polymerase chain reaction (PCR) on a CFX-96 amplifier (Bio-Rad, USA). Used reagent kits (Litekh, Moscow) in accordance with the instructions

Results: A positive association of Ala54Thr with body mass index, blood pressure level was revealed. In 50% of cases, patients were diagnosed with GG SNP (Ala/Ala), in 28% of cases - GA SNP (Ala/Thr), in 22% - AA SNP (Thr/Thr). Indicators of glycemic control, levels of triglycerides and low density lipoproteins were significantly higher in patients with the AA (Thr/Thr) group. In the same group, a lower ankle-brachial index was noted (P = 0.04).

Conclusions: AA SNP genotype (Thr/Thr) is associated with an increased incidence of peripheral atherosclerosis in patients with type 2 diabetes.