SFEBES2021 Poster Presentations Metabolism, Obesity and Diabetes (78 abstracts)
The Effect of Bariatric Surgery on the proteome of people achieving remission of Type 2 Diabetes
Zohaib Iqbal 1 , Helene Fachim 1 , John Gibson 2 , Ivona Baricevic-Jones 1 , Amy Campbell 1 , Bethany Geary 1 , Rachelle Donn 1 , Dashne Hamarashid 2 , Akheel Syed 2 , Anthony Whetton 1 , Handrean Soran 3 & Adrian Heald 2
1University of Manchester, Manchester, United Kingdom; 2Salford Royal Foundation Trust, Manchester, United Kingdom; 3Manchester Foundation Trust, Manchester, United Kingdom
Background: Bariatric surgery (BS) results in metabolic pathway recalibration. When major metabolic change occurs, blood protein components have a key role and can be altered significantly. We set out to identify potential biomarkers of change in plasma following BS in people achieving remission of type 2 diabetes mellitus (T2DM).
Methods: Longitudinal analysis was performed on serum samples from 10 individuals who all achieved remission of T2DM following Roux-en-Y gastric bypass (n = 7) or Sleeve gastrectomy (n = 3). Sequential window acquisition of all theoretical fragment ion spectra Mass Spectrometry (SWATH-MS) was on serum samples taking at 4 months before and 6 and 12 months after BS.
Results: 467 proteins were quantified by SWATH-MS. Principal component analysis resolved samples from distinct time points after selection of key discriminatory proteins: Twenty-five proteins were differentially expressed between pre-surgery and 6 months post-surgery; thirty-nine proteins between baseline and 12 months. Eight proteins were significantly different to pre-surgery samples at both 6- and 12-months post-surgery. These were: sex hormone binding globulin(SHBG), Serotransferrin(TF), Proteoglycan 4, Apolipoprotein A4(APOA4), Leucine-rich alpha-2-glycoprotein, Heat shock 70 kDa protein 4(HSPA4), Bifunctional epoxide hydrolase 2 and N-acetylmuramoyl-L-alanine amidase. The panel of proteins identified as consistently different, included peptides related to insulin sensitivity (SHBG increase)(FC: Fold change 12 months vs baseline), p value)(1.95, P < 0.01), systemic inflammation (TF and HSPA4 – both decreased) (TF Fold change 12 months vs baseline -0.78, P < 0.01; HSPA4 Fold Change 12 months vs baseline -0.38, P < 0.05), and lipid metabolism (APOA4 decreased) (APOA4 Fold Change 12 months vs baseline -1.38, P < 0.05).
Conclusion: Using the technique of SWATH-MS to generate proteomic maps, we have shown significant change in serum protein levels for a number of metabolically relevant proteins from pre-BS to 6- and 12-months post-surgery. Several of these proteins are key components in critical metabolic and inflammatory pathways.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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