SFEBES2021 Poster Presentations Metabolism, Obesity and Diabetes (78 abstracts)
N-acetylmuramoyl-L-alanine amidase is a biomarker for remission of type 2 diabetes after bariatric surgery
Zohaib Iqbal 1 , Helene Fachim 1 , John Gibson 2 , Ivona Baricevic-Jones 1 , Amy Campbell 1 , Bethany Geary 1 , Rachelle Donn 1 , Akheel Syed 2 , Anthony Wetton 1 , Handrean Soran 3 & Adrian Heald 2
1University of Manchester, Manchester, United Kingdom; 2Salford Royal Foundation Trust, Manchester, United Kingdom; 3Manchester Foundation Trust, Manchester, United Kingdom
Aims: We used sequential window acquisition of all theoretical fragment ion spectra Mass Spectrometry (SWATH-MS) to identify proteins acting as markers of remission of type 2 diabetes (T2DM) in patients who lost weight after bariatric surgery.
Background: Bariatric surgery results in remission of T2DM in up to 80% of patients. The mechanisms underpinning are largely unknown. N-acetylmuramoyl-L-alanine amidase (PGLRYP2) is an immune response enzyme that breaks down glycopeptides, with a principal role in fighting bacterial infection. It is expressed constitutively from hepatocytes and previous proteomic studies have suggested utility in diagnosing sepsis. No studies have examined the role this enzyme plays in T2DM.
Methods: Longitudinal analysis was performed on plasma samples from ten individuals who achieved remission of T2DM post Roux-en-Y gastric bypass (n = 7) or Sleeve gastrectomy (n = 3). SWATH MS was performed on baseline/6 month/12 month samples.
Results: SWATH-MS identified and quantified 467 proteins post-surgery. Twenty-five proteins were differentially expressed between pre-surgery and 6 months post-surgery; 39 proteins between baseline and 12-months. Eight proteins were significantly different at both 6-and 12-month time points. These are listed in Table 1. The fold change of PGLYRP2 showed a significant negative correlation with change in HbA1c at 6-months and at 12-months and change in BMI at 12-months.
| Table 1 | Log FC 6-Months Vs Baseline | Log FC 12 Months Vs Baseline | P Value |
| Serotransferrin | -1.0677252 | -0.7764836 | P < 0.001 |
| Proteoglycan 4 | -0.8979797 | -0.77934 | P < 0.05 |
| Sex hormone-binding globulin | 1.48545057 | 1.95102943 | P < 0.05 |
| Bifunctional epoxide hydrolase 2 | -0.4118723 | -0.4725174 | P < 0.05 |
| Apolipoprotein A-IV | -0.7125427 | -1.3811785 | P < 0.05 |
| PGLYRP2 | 0.39129906 | 0.43772459 | P < 0.05 |
| Heat shock 70 kDa protein 4 | -0.4967544 | -0.3887322 | P < 0.05 |
| Leucine-rich alpha-2-glycoprotein! | 0.5324538 | 0.5912797 | P < 0.05 |
Conclusions: Using SWATH-MS we identified significant changes in PGLYRP2 which correlated with both BMI and HbA1c consistently, suggesting a role for this protein in the remission of T2DM post BS which warrants further investigation.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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