SFEBES2021 Poster Presentations Adrenal and Cardiovascular (45 abstracts)
Classic and 11-oxygenated androgens in serum and saliva across adulthood and the menstrual cycle – a mass spectrometry-based cross-sectional study
Lina Schiffer 1 , Punith Kempegowda 1 , Joanne E Adaway 2 , Fozia Shaheen 1 , Andreas Ebbehoj 3 , Sumitabh Singh 3 , Alessandro Prete 1 , James Hawley 1,2 , Alice J. Sitch 4 , Brian G. Keevil 2 , Irina Bancos 3 , Angela E. Taylor 1 & Wiebke Arlt 1
1Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, United Kingdom; 2Department of Clinical Biochemistry, Wythenshawe Hospital, Manchester, United Kingdom; 3Division of Endocrinology, Metabolism, Diabetes and Nutrition, Department of Internal Medicine, Mayo Clinic, Rochester, USA; 4Institute of Applied Health Research, University of Birmingham, Birmingham, United Kingdom
Background: The gonads are the major source of classic androgens during reproductive years. Additionally, the adrenal gland produces precursors for both classic and 11-oxygenated androgen biosynthesis, with androgen activation predominantly occurring in peripheral target tissues of androgen action. We used liquid chromatography-tandem mass spectrometry to profile classic and 11-oxygenated androgens in serum and saliva across the adult age range and assessed diurnal as well as menstrual cycle-dependent variation.
Methods: We collected morning serum samples from 294 healthy volunteers (126 men, 22-95 years; 168 women, 21-91 years, 91 post- and 77 premenopausal, 16 on combined oral contraceptives, COCP). Morning saliva was collected by 83 healthy volunteers (51w, 32m); 26 volunteers (13w, 13m) also collected a 7-timepoint diurnal saliva profile and 12 women collected diurnal profiles during both follicular and luteal phase as well as morning saliva on 7 consecutive days during the follicular and luteal phase, respectively. Samples were profiled by liquid chromatography-tandem mass spectrometry (serum: 25 steroids; saliva: 6 steroids).
Results: In serum, classic androgen pathway steroids (DHEA, DHEAS, androstenedione, testosterone, dihydrotestosterone) decreased with age in both men and women. By contrast, serum 11-hydroxyandrostenedione and 11-ketotestosterone remained constant with age. Of note, in both sexes 11-ketoandrostenedione decreased with age and 11-hydroxytestosterone increased, in keeping with altered peripheral metabolism due to an age-dependent increase in HSD11B1 activity. Women on COCP had lower androstenedione, testosterone and 11-ketotestosterone concentrations. In saliva, classic and 11-oxygenated androgens showed a clear diurnal pattern in men and in the follicular phase in women, but only 11-oxygenated androgens showed luteal phase diurnal variation. Classic androgens were higher in the luteal phase while 11-oxygenated androgens remained unchanged across the menstrual cycle.
Conclusions: 11-oxygenated androgens form a stable pool during adulthood while classic androgens decline with age and are subject to menstrual cycle-dependent variation.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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