SFEBES2021 Oral Poster Presentations Metabolism, Obesity and Diabetes (4 abstracts)
Investigating the role of the gut metabolome in appetite and obesity
Hannah Stephens 1 , Anya Ramgulam 1 , Georgia Franco-Becker 1 , Martina Tashkova 1,2 , Jose Ivan Serrano Contreras 1 , Dominic Blunt 2 , Isabel Garcia-Perez 1 , Gary Frost 1 & Kevin Murphy 1
1Imperial College London, London, United Kingdom; 2Imperial College Healthcare NHS Trust, London, United Kingdom
Overweight and obesity is an escalating global health problem, affecting 40% of the population and being the 5th largest cause of death worldwide. Treatment options are limited, with pharmaceutical approaches being inadequate and the success of bariatric surgery being limited by its invasive nature. The regulation of appetite by small bioactive compounds in the gastrointestinal tract is an important target in antiobesity research. Metabolites from diet and both host and microbial metabolism interact with G protein-coupled receptors in the gut epithelium, triggering the release of hormones that act on the appetite regulatory centres in the brain to promote satiety. Understanding how gut metabolites influence appetite could lead to novel antiobesity strategies. Here, 10 participants (Age 47.40 ± 3.70 years, body mass index 25.69 ± 0.84 kg/m2) attended the clinical research facility for a 4-day inpatient stay. Nasoenteric tubes were inserted to allow sampling of gut content from the distal ileum and the proximal colon. Following ingestion of a high-protein and high-fibre test meal (695 kcal, 55% protein, 27g fibre), gut samples were collected every 30 minutes for 6 h. Visual analogue scales were used to measure subjective appetite, and blood samples drawn for the measurement of appetite hormones. Comprehensive 1H-NMR metabolomic analysis provided novel information about the metabolic environment of different regions of the intestine that accompanies the enhanced satiety from this type of meal. Analysis showed distinct metabolite processing over a timeline from baseline until 360 minutes after ingestion, including perturbations to various amino acids, such as isoleucine, leucine and aspartate, carbohydrates such as glucose, sucrose and lactose, and bile acids such as cholic acid. The correlation of these NMR datasets with appetite and gut hormone data revealed important information about the role of nutrient sensing in appetite regulation, which could have implications in the treatment of obesity.
Volume 77
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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