Endocrine Abstracts

Purpose of research

To determine the level of insulin content, the content of nitric oxide metabolites, Malondialdehyde, the activity of lipoprotein-associated phospholipase A2 (FLA2), in the blood serum of patients with NFLD with type II D and impaired glucose tolerance.

Material and methods

The study included 74 patients with non-alcoholic fatty liver disease. 30 of them have diabetes of the second type, and 34 patients have impaired glucose tolerance. The average age of patients is 55.6 ± 2.2 years. Body mass index is 34.85 ± 1.79 kg/m². Phospholipase A2 was determined by chemiluminescent immunological analysis on the device ’Siemens’. Fatty acids were determined by enzyme method using test-systems (England). Metabolites of nitric oxide were determined by the method Metel’skaia V. A., Malonicdialdehyde (MDA) was determined by the method of Andreeva.

Research result

Insulin levels were determined in all 74 patients. Hyperinsulinemia was observed in 30 patients with low glucose tolerance. 34.65 ± 4.16 mkme/ml. Readings in the control group total 8.3 ± 1 mkme/ml. In patients with diabetes, the insulin content was 6.28 ± 0. 4 (P = 0.001), which caused the expression of phospholipase A2 3 times in diabetes and twice with low glucose tolerance. In non-alcoholic fatty liver disease activity of nitric oxide and its content increased. The level of metabolites increased in parallel with the activity of alanine Aminotransferase. The content of malonic aldehyde in patients with diabetes increased and made up 24.12 + - of 1.64 mmol/l. patients with low tolerance to glucose the content of MDA made up 16.91 ± 3.87. Monitoring indicators equal to 9.94 ± 1.62 µmol/l, P = 0.001. A negative correlation between insulin and Malon aldehyde was revealed.(r = -0.31). The interrelation of the lipid peroxidation and leading to phospholipase mechanism of damage to the lipid membranes of hepatocytes FLA2 and MDA r = -0.53; P = 0.001; between NO and FLA2 = 0.625. P = 0.001.

Summary

High activity of inflammation markers in patients with NAND with type 2 diabetes is a diagnostic and prognostic criterion for the activity of the inflammatory process in the liver with a high risk of complications. Hyperglycemia induces the production of free radicals, reduces the activity of nitric oxide synthase and disrupts the sensitivity of insulin receptors.