Endocrine Abstracts

Introduction

NaKATPase is membrane bound enzyme, necessary for maintenance of the cell potential and volume, ion flow, neuronal signal transduction. Previous studies showed decreasing of its activity in patients with diabetes mellitus (DM). Mechanisms regulated enzymes activity in DM is not clear yet. We proposed that glycemia is a main regulator of NaKATPase activity in erythrocytes at the DM.

Material and methods

In 76 patients with DM, 31 with DM type1 and 36 with DM type 2. In observed persons blood glycemia, HbA1c, blood plasma tryglycerides, cholesterol level were measured. Erythrocytes separated from blood, washed out with buffer saline. NaKATPase activity measured by differences of the inorganic phosphorus level in media after incubation with ATP.

Results

Erythrocytes NKATPase activity were decreased by 25% in patients with DM1 and 29.9% (P < 0.05) in patients with DM2 (P < 0.05) compared with healthy subjects. Enzymes activity were not depends from age and gender in person younger than 40 years old. There were no any differences in enzymes activity between patients with DM type 1 and type 2. Blood glycemia, HbA1c, tryglycerides and cholesterol levels were significantly higher in patients with DM in compare with healthy subjects and suggest about metabolic disturbances. Erythrocytes NaKATPase activity shown negative correlation with glycemia and HbA1c level. Interestingly, NaKATPase activity significantly increased in those who achieved better glycemic control, whereas remain unchanged in those with poor glycemia.

Conclusion

Erythrocytes NaKATPase activity is decreased in patients with DM type 1 and 2, and negatively depends from glycemia and HbA1c level. Glycemia is an independent factor in regulation of erythrocytes NaKATPase activity.