ECE2021 Audio Eposter Presentations Reproductive and Developmental Endocrinology (55 abstracts)
Effects of mitochondrial dynamics during myocardial differentiation in 3D model
Sunhwa Jeong 1 , Minsu Lee 1 , Seon Mi Park 1 , Jimin Lee 1 & Eui-Bae Jeung 1
1Laboratory of Veterinary Biochemistry and Molecular Biology, Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Veterinary Biochemistry and Molecular Biology, Cheongju, Republic of South Korea
Mitochondria, which are essential organelles for endocrine health, plays an important role in various physiological functions including hormonal biosynthesis, cell metabolism, proliferation and differentiation. Thus, mitochondrial toxicity can affect a variety of organs, such as liver, heart, muscle, kidney, and central nervous system. Mitochondrial toxicity is recognized as a contributor to drug-induced toxicity of various drugs such as hydroxytamoxifen, valproic acid, acetaminophen, doxorubicin, and amiodarone. Here we describe in vitro assay using 3D culture in embryonic body (EB) state and establish a novel model of idiosyncratic toxicity based on mitochondrial impairment. The EBs were cultured in 3D model, and exposed to chemicals for 4 days. This study aims to investigate mitochondrial dynamics during myocardial differentiation in mouse embryonic stem cells(mESCs). In the result, ID50 values under 10-3M each toxic chemical in EB state were obtained; the expression levels of mRNA associated mitochondrial complex were decreased in high concentration group. We also compared mitochondrial DNA copy numbers to examine the effect on the number of mitochondria in mESCs-derived cardiomyocytes. Taken together, the results suggest that mitochondrial toxicity of unknown chemicals can be estimated with our alternative in vitro assay.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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