ECE2021 Audio Eposter Presentations Diabetes, Obesity, Metabolism and Nutrition (223 abstracts)
Amniotic membrane treatment of an oxidative stress murine chronic diabetic wound model
Adriana Panayi 1 , Mengfan Wu 1 , Qinxin Liu 2 , Mehran Karvar 1 , Shimpo Aoki 1 , Yori Endo 1 , Chenhao Ma 3 , Ryoko Hamaguchi 1 & Dennis Orgill 1
1Harvard Medical School, Boston, United States; 2Tongji Medical College of Huazhong University of Science and Technology College of Medicine and Health Management, Wuhan Shi, China; 3Peking Union Medical College Hospital, China
Background
To date, available animal models have failed to replicate the complex pathophysiology of chronic diabetic wounds. In a recent study we administered a regimen of antioxidant enzyme inhibitors (AIE) to develop a reliable and effective murine model. Here we use this model to test the efficacy of an amniotic membrane (AM) scaffold.
Methods
30 db/db mice received a pre-established AIE regimen and a 1 × 1 cm2 full thickness skin wound was excised on their dorsum. The wounds of 15 mice were covered with AM and occlusive dressing (AM group). The remaining 15 received only occlusive dressing (Blank group). The wounds were photographed on day 0, 10, 14, 21 and 28 and mice were sacrificed at various time points with the tissue harvested for analysis.
Results
Histologically, the mice in the AM group had higher wound bed thickness, collagen deposition and keratinocyte proliferation than the Blank mice. The amniotic membrane appeared to improve the vascularization of the wound, both in terms of density and maturity of the vessels. Leucocyte infiltration was comparable between the groups and the wound healing rate of the AM group was similar to the untreated mice.
Conclusions
The AIE regimen arrested the wounds in the inflammatory stage of healing while treatment with amniotic membrane offers promising potential. Although the wound healing rate between the two groups was comparable, the scaffold improved the overall quality of the wound elucidating the mechanisms of function of the scaffold on chronic wound healing. Better understanding of the mechanisms of healing of chronic wounds will be instrumental for the development of more effective treatments.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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