ECE2020 ePoster Presentations Diabetes, Obesity, Metabolism and Nutrition (142 abstracts)
1Institute of Biophysics and Biochemistry, Mirzo Ulugbek National University of Uzbekistan, Biochemistry, Tashkent, Uzbekistan; 2Institute of Hematology and Blood Transfusion, Uzbekistan Public Healthcare Ministry, Molecular Medicine and Cell Technologies, Tashkent, Uzbekistan; 3Ya.Kh. Turakulov Center for the Scientific and Clinical Study of Endocrinology, Uzbekistan Public Healthcare Ministry, Tashkent, Uzbekistan
The fat tissue inflammation and insulin resistance formation are common in obesity and type 2 diabetes mellitus. The tumor necrosis factor–α (TNF-α), a pro-inflammatory cytokine, was found to be a key one in onset and progression of insulin resistance, blocking the insulin signaling pathway and hampering the gene expression of GLUT4, the intracellular insulin-regulated glucose transporter in body tissues. Rise in the TNF-α concentration in blood serum is believed to be associated with the excessive body mass or obesity. TNF-α can be used for diagnosis of diabetes mellitus in the obese patients. The work was initiated to identify associations between TNF-α blood serum levels in the obese patients and risk of diabetes mellitus onset. There were 3 groups of persons examined. The persons with normal body mass index (BMI) were included into the 1st group, the non-diabetic obese persons with BMI more than 28 kg/m2 and the diabetic obese persons were included into the 2nd and 3rd group, respectively, for the anthropometric and clinical-laboratory parameters of the carbohydrate and lipid metabolism to be measured by the enzyme-linked immunosorbent assay (ELISA). The MR-96A Microplate Reader (Shenzhen Mindray Bio-Medical Electronics Co. Ltd., China) was used to perform qualitative or quantitative determination of samples by the photometric measurements. The findings demonstrated that serum TNF-α levels in the non-diabetic obese persons (3.55 ± 0.30 pg/ml) and the obese diabetics (3.96 ± 0.14 pg/ml) were significantly higher than those in the controls (0.79 ± 0.30 pg/ml) and mean standard reference values. Measurements of TNF-α levels in blood sera of the obese persons are important for early diagnosis of main metabolic disorders resulting in T2DM onset.