ECE2020 ePoster Presentations Diabetes, Obesity, Metabolism and Nutrition (142 abstracts)
Moscow Clinical Research and Practice Center Named After Loginov, Moscow, Russian Federation
Glycation is due to the ability of glucose to form irreversible chemical compounds with amino groups of lysine and arginine – methylglyoxal (MG).
Material and Methods: 98 patients with NAFLD and 52 patients with NAFLD were examined. Diabetes 2-type, and 22 healthy volunteers. Age 57.3 ± 5.2 years. BMI 34.85 ± 1.79. The diagnosis was established on the basis of laboratory data and examination results. The level of LPO was determinedby the content of malonic dialdehyde (MDA).
Results: In patients with NAFLD, the content of MG in the blood serum520.75 ± 114.35, in the control-69.02 ± 6.67 nm/l (P = 0.001). In T2DM patients the MG content was 292.11 ± 16.34 nm/l. Observed correlation of MG and MDA (r = 0.495).
Conclusions: Patients with NAFLD have a significant increase in MG b serum 7 times compared to the control. MG damage arginine residues of proteins, disrupts the transmission of the insulin signal, inhibits enzymes and plays a key role in the development of insulin resistance and hyperglycemia. Quantitative determination of MG in serum by HPLC method can be used as a prognostic and diagnostic test glucotoxicity.
Keywords: NAFLD, type 2 diabetes, glycosylation, methylglyoxal.