ECE2020 Audio ePoster Presentations Hot topics (including COVID-19) (110 abstracts)
The effects of selective activation of kisspeptin neurons in hypothalamic arcuate nucleus on energy metabolism in mice
Qian Li 1 , Qinyu Liu 1 , Guifeng Huang 1 , Wei Lin 1 , Gang Chen 1,2 & Junping Wen 1
1Department of Endocrinology, Fujian Medical University, Fujian Medical University, China; 2Fujian Key Laboratory of Medical Measurement, Fujian Academy of Medical Sciences
Objectives: This study aims to determine which metabolic tissue is involved in the process of kisspeptin neurons regulate energy metabolism so that it may provide a novel therapy for metabolic related diseases such as obesity.
Methods: Kiss1-CreGFP mice were used in this study. And we selectively activated kisspeptin neurons by using Designer-receptors-exclusively-activated-by-designer-drugs (DREADDs) technology. Mice were bilaterally injected with the Cre-dependent adeno-associated viral (AAV) vector encoding human M3-muscarinic receptor (hM3Dq) fused with red fluorescent protein in ARC. And peripheral administration with clozapine N-oxide (CNO) leads to CNO-dependent activation of kisspeptin neurons in ARC (kiss1ARC), then, we studied metabolic phenotype of mice.
Results: Neuronal activation of kisspeptin neurons with DREADDs decreases body weight, daily average food intake, and increases energy expenditure by altering brown adipose tissue (BAT) thermogenic process and browning of white adipose tissue (WAT), which is depend on different energy states only in female mice.
Conclusions: Our results previously demonstrated that kisspeptin neurons play crucial role in controlling energy metabolism, which is sexually dimorphic.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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