ECE2020 ePoster Presentations Diabetes, Obesity, Metabolism and Nutrition (142 abstracts)
Study on effect of the tumor necrosis factor–α on onset and progression of obesity in patients with diabetes mellitus
Sanobarkhon Irgasheva 1 , Zafar Ibragimov 1 , Elvira Ibragimova 1 , Timur Alimov 2 , Nasiba Alimova 3 , Nigora Maksutova 3 , Khamid Karimov 2 , Tokhir Ishanhodjaev 1 & Talat Saatov 1
1Institute of Biophysics and Biochemistry, Mirzo Ulugbek National University of Uzbekistan, Biochemistry, Tashkent, Uzbekistan; 2Institute of Hematology and Blood Transfusion, Uzbekistan Public Healthcare Ministry, Molecular Medicine and Cell Technologies, Tashkent, Uzbekistan; 3Ya.Kh. Turakulov Center for the Scientific and Clinical Study of Endocrinology, Uzbekistan Public Healthcare Ministry, Tashkent, Uzbekistan
The fat tissue inflammation and insulin resistance formation are common in obesity and type 2 diabetes mellitus. The tumor necrosis factor–α (TNF-α), a pro-inflammatory cytokine, was found to be a key one in onset and progression of insulin resistance, blocking the insulin signaling pathway and hampering the gene expression of GLUT4, the intracellular insulin-regulated glucose transporter in body tissues. Rise in the TNF-α concentration in blood serum is believed to be associated with the excessive body mass or obesity. TNF-α can be used for diagnosis of diabetes mellitus in the obese patients. The work was initiated to identify associations between TNF-α blood serum levels in the obese patients and risk of diabetes mellitus onset. There were 3 groups of persons examined. The persons with normal body mass index (BMI) were included into the 1st group, the non-diabetic obese persons with BMI more than 28 kg/m2 and the diabetic obese persons were included into the 2nd and 3rd group, respectively, for the anthropometric and clinical-laboratory parameters of the carbohydrate and lipid metabolism to be measured by the enzyme-linked immunosorbent assay (ELISA). The MR-96A Microplate Reader (Shenzhen Mindray Bio-Medical Electronics Co. Ltd., China) was used to perform qualitative or quantitative determination of samples by the photometric measurements. The findings demonstrated that serum TNF-α levels in the non-diabetic obese persons (3.55 ± 0.30 pg/ml) and the obese diabetics (3.96 ± 0.14 pg/ml) were significantly higher than those in the controls (0.79 ± 0.30 pg/ml) and mean standard reference values. Measurements of TNF-α levels in blood sera of the obese persons are important for early diagnosis of main metabolic disorders resulting in T2DM onset.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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