Endocrine Abstracts

Glycation is due to the ability of glucose to form irreversible chemical compounds with amino groups of lysine and arginine – methylglyoxal (MG).

Material and Methods: 98 patients with NAFLD and 52 patients with NAFLD were examined. Diabetes 2-type, and 22 healthy volunteers. Age 57.3 ± 5.2 years. BMI 34.85 ± 1.79. The diagnosis was established on the basis of laboratory data and examination results. The level of LPO was determinedby the content of malonic dialdehyde (MDA).

Results: In patients with NAFLD, the content of MG in the blood serum520.75 ± 114.35, in the control-69.02 ± 6.67 nm/l (P = 0.001). In T2DM patients the MG content was 292.11 ± 16.34 nm/l. Observed correlation of MG and MDA (r = 0.495).

Conclusions: Patients with NAFLD have a significant increase in MG b serum 7 times compared to the control. MG damage arginine residues of proteins, disrupts the transmission of the insulin signal, inhibits enzymes and plays a key role in the development of insulin resistance and hyperglycemia. Quantitative determination of MG in serum by HPLC method can be used as a prognostic and diagnostic test glucotoxicity.

Keywords: NAFLD, type 2 diabetes, glycosylation, methylglyoxal.