ECE2020 Audio ePoster Presentations Thyroid (144 abstracts)
Evaluation of miRNA-222, –146b, –181b as possible diagnostic markers in patients with papillary thyroid carcinoma
Aiste Kondrotiene 1 , Daina Pamedytyte 2 , Vaida Simanaviciene 2 , Dalia Dauksiene 1 , Aurelija Zvirbliene 2 , Mintaute Kazokaite 1 , Raimonda Klimaite 1 , Valdas Sarauskas 3 , Albertas Daukša 4 , Rasa Verkauskiene 1 & Birute Zilaitiene 1
1Institute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania; 2Institute of Biotechnology, Vilnius University, Vilnius, Lithuania; 3Department of Pathology, Lithuanian University of Health Sciences, Kaunas, Lithuania; 4Institute of Digestive Research, Medical Academy, Faculty of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania
Introduction: The sensitivity and specificity of biomarkers which have been used in clinical practice for diagnosis of papillary thyroid carcinoma (PTC) are low. It is essential to develop novel diagnostic biomarkers for PTC.
Objective: We aimed to explore the expression levels of miR-222, – 146b, –181b in PTC tissue and plasma samples and to compare with benign thyroid nodules (BTN).
Methods: We collected plasma samples from the patients just before the thyroidectomy due to PTC or BTN. Tissue samples were also collected from the subgroups of these patients. The expression levels (2^-∆Ct) of plasma miR-222, –146b, –181b were measured in 48 samples from patients with PTC and 22 from patients with BTN. Expression of the same set of miRNAs in PTC and BTN tissue samples were investigated in subgroups of 19 and 9 patients, respectively.
Results: A significantly higher expression levels of miR-146b were observed in PTC tissue samples (4.433 ± 6.04), compared to BTN (0.151 ± 0.094), (P = 0.001). However, the results of plasma samples indicated that levels of miR-222, –146b, –181b had no significant differences in the PTC patients compared with BTN (P = 0.424, P = 0.641, P = 0.427, respectively).
Conclusion: Many studies are underway to discover minimally invasive method for PTC diagnostics. In our study we analyzed expression levels of miR-146b, –222, –181b and we found that only mir-146b expression levels were significantly higher in PTC than in BTN tissue samples. However, there were no significant differences while comparing expression levels of this set of miRNAs in PTC and BTN plasma samples. Unfortunately, the value of these three miRNAs is still limited in differential diagnosis of PTC and BTN from plasma samples.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more