Endocrine Abstracts

Kisspeptin (metastin, HH13, KiSS-1, KiSS-1 metastasis-suppressor, KiSS-1 metastasis suppressor) is a neuropeptid that is encoded by the KISS1 gene (Messager et al., 2005). Kisspeptin-GPR54 signaling has an significant role in initiating secretion of gonadotropin-releasing hormone at puberty (K. Skorupskaite et al., 2014). Is known that kisspeptin to play a role in tumor suppression (especially in breast tissue), (E.J. Mead et al., 2007).

Objectives: To determine kisspeptin levels in healthy children and adolescents, in children and adolescents with endocrine and mammalogic diseases.

Patients and Methods: Serum kisspeptin levels was determined in 123 children and adolescents (aged 0.7–18 yrs), in the 8 groups, including control groups. The healthy children was 12, healthy adolescents – 30, children with central precocious puberty (precocious puberty CPP and premature isolated the larche PT) – 11, girls with amenorrhea – 23, adolescent girls with dyshormonal mammary dysplasia – 12, patients with polycystic ovary syndrome (POS) – 9, pregnant adolescent girls – 15 and adolescents with gynecomastia was 11 boys. The concentration of kisspeptin was measured kisspeptin (total) using competitive enzyme immunoassay. Results were analyzed using Pearson’s chi-squared test. Data are expressed as median, P value of < 0.05 was considered statistically significant. This study was carried out in accordance with the Helsinki Declaration.

Results: The investigation shows that healthy and pregnant adolescent girls, boys with gynecomastia had kisspeptin median level was 30 pg/ml. Among the girls with amenorrhea kisspeptin level was lower 10 pg/ml (P = 0.01). Girls with mastopathy (dyshormonal dysplasia) had kisspeptin level 50 pg/ml (P = 0.03). Serum kisspeptin levels were significantly higher in children with CPP and PIT and adolescent girls with polycystic ovary syndrome than in control group (80 and 300 vs 30 pg/ml, P = 0.01 and P < 0.01).

Conclusions: Kisspeptin level was various in healthy children, girls with amenorrhe, mastopathy, polycystic ovary syndrome, premature isolated thelarche, children with central precocious puberty, pregnant adolescent girls and boys with with gynecomastia. In this study data received that serum kisspeptin level was significantly lower in girls with amenorrhea and higher in children with CPP and PT, girls with mastopathy and POS. Kisspeptin levels in girls with mammological diseases are statistically significantly higher, which can be explained by compensatory reactions in response to proliferation in mammary glandular tissue. The serum kisspeptin level may be used as a biomarker of sexual disorders, polycystic ovary syndrome and mammary diseases and in girls.