ECE2020 Audio ePoster Presentations Bone and Calcium (121 abstracts)
Serum and tissue expression profile of microRNAs that regulate genes related to the pathogenesis of sporadic parathyroid adenomas
Maria Yavropoulou 1 , Kalliopi Pazaitou-Panayiotou 2 , Polyzois Makras 3 , Athanasios Anastasilakis 4 , Chris Poulios 5 , Emily Daskalaki 5 , Sofia Chrysafi 5 , Kyriakos Vamvakidis 6 , Dimitris Vlachodimitropoulos 7 & John Yovos 8
1National and Kapodistrian University of Athens, Endocrinology Unit, 1st Department of Propedeutic Internal Medicine, Athens, Greece; 2Interbalkan Medical Center, Thessaloniki, Greece; 3251 Hellenic Air Force & VA General Hospital, Department of Medical Research, Athens, Greece; 4424 General Military Hospital, Department of Endocrinology, Thessaloniki,; 5Faculty of Medicine, Aristotle University of Thessaloniki, Pathology Department, Thessaloniki, Greece; 6Henry Dunant Hospital Center, Department of Endocrine Surgery, Athens, Greece; 7Medical School, National and Kapodistrian University of Athens, Laboratory of Forensic Medicine and Toxicology, Athens, Greece; 8School of Medicine, Aristotle University of Thessaloniki, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
Background: Epigenetic changes appear to be implicated in the development of parathyroid tumors. In this study we investigated the microRNA expression profile in the serum and tissue samples from patients with primary hyperparathyroidism (PHP) due to sporadic parathyroid adenomas (SPAs).
Methods: Our study cohort consisted of 40 patients with PHP due to SPAs that underwent parathyroidectomy (PTX).
MicroRNA extraction was performed from a) 40 FFPE samples of sPA, b) 10 FFPE samples of normal parathyroid tissue (NPT) from patients that underwent total thyroidectomy for benign multinodular goiter, c) serum samples from the 40 cohort patients with PHP at two time points (before and 2 months post PTX), d) serum samples from 10 healthy individuals that served as controls, also at two time points (t1 = baseline and t2 = 2 months after). Nine microRNAs (miRs) were selected based on their interaction with genes related to the pathogenesis of sporadic parathyroid adenomas (namely, miR-17-5p, miR-24, miR-29b, miR-31, miR-135b-5p, miR-186, miR-195, miR-330-3p, and miR-483-3p)
Results: The microRNA expression profile of SPAs at tissue level differed significantly compared to NPT. In particular, the relative expression of 4 miRs, namely miR-17-5p, miR-31, miR-135 and miR-186 was significantly decreased in SPAs compared to NPT (fold change 0.17, fold change 0.034, fold change 0.01 and fold change 0.09, respectively, all P values<0.001). On the other hand, the relative expression of 2 of the tested miRs was significantly increased in SPAs (miR-24, fold change 12.4, P < 0.001; miR-29b, fold change 18.5, P = 0.011). Similar to the microRNA profile in the tissue the relative expression of miR-135 was also significantly decreased in the serum of patients with PHP compared to controls (fold change 00.7, P < 0.001), while no significant differences were found in the serum of PHP patients before and after parathyroidectomy.
Conclusion: MicroRNAs that regulate genes linked to the pathogenesis of SPAs, such as menin 1 (miR-24 and miR-29b), cyclin D1 (miR-17-5p) and CaSR (miR-31 and miR-135) are significanlty deregulated in SPA samples compared to NPT, suggesting a role for epigenetic changes in the development f SPAs.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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