ECE2019 Poster Presentations Reproductive Endocrinology 2 (39 abstracts)
AdipoRon, an adiponectin receptor agonist, reduces the proliferation and secretion of steroids by human granulosa cells
Jeremy Grandhaye 1 , Ingrid Plotton 2 , Sandy Hmadeh 1 , Alice Bongrani 1 , Remy LeGuevel 3 , Christelle Ramé 1 , Joelle Dupont 1 & Pascal Froment 1
1INRA Centre Val de Loire, Nouzilly, France; 2Molecular Endocrinology and Rare Diseases, University Hospital, Claude Bernard Lyon 1 University, Bron, France; 3Plate-forme ImPACcell, Université de Rennes 1, Rennes, France.
Diet, and especially metabolic dysregulation such as obesity can influence the ovarian activity. Adipose tissue plays an important role since it is known to be one of the most important endocrine organs producing hundreds of cytokines, such as adiponectin. Our team has observed that adiponectin modulated steroid secretion by the ovarian granulosa cells and its concentration in the follicular fluid was influenced by the metabolic status. Recently, a small adiponectin mimetic synthetic molecule, called AdipoRon, has been developed. In this study, we have the objective to study its role on human granulosa cells. The experiments were performed on the KGN cell line, a human granulosa line (Nishi et al., 2001), as well as on primary granulosa cells prepared from IVF protocol. The cells were exposed to AdipoRon (2.5 μM and 25 μM) for 48h and 96 h. The dose of 25 μM has been described in the literature and observed in the laboratory, as being activator of the adiponectin pathway (10 μg/ml). After 96 hours of AdipoRon (25 μM) exposure, primary granulosa cells and KGN showed a 30% reduction in cell proliferation with no change in cell mortality, observed by BrDU incorporation and PCNA protein expression. AdipoRon also modified cell metabolism (lactate increase), reduction in steroid secretion and in the Reactive Oxygen Species production, which is a marker of cellular stress. In conclusion, these results indicate that AdipoRon is able to modify the metabolism of granulosa cells and could be a potential compound in the development of new therapeutic strategies requiring the reduction in cell proliferation or steroid production of the granulosa cells.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more