Endocrine Abstracts

Background and aims: Comparative animal study of efficacy of intermittent short courses administration of lyophilized single-, three- and alive multistrain probiotic on insulin resistance in rats with experimental obesity.

Methods: We included 70 rats divided into 7 groups (n=10 in each). Rats of group I were intact. Newborn rats of groups II-VII were injected with monosodium glutamate (MSG) (4 mg/g). Rats of group II (MSG-obesity group) were untreated. The groups III-V received lyophilized mono-probiotics B.animalis VKL, B.animalis VKB, L.casei IMVB−7280 respectively. The group VI received the mix of these three probiotic strains. The group VII was treated with multi-probiotic ‘Symbiter’ which contains 14 alive probiotic strains (Lactobacillus, Bifidobacterium, Propionibacterium, Acetobacter genera).

Results: Neonatal treatment with MSG lead to the development of obesity in all MSG-obesity rats and up to 20–70% after probiotic administration. Supplementation of probiotic composition, with preference to alive strains (group VII), led to a significantly lower prevalence of obesity, decreasing of HOMA-IR (2.31±0.13 vs 3.07±0.3; P<0.001), proinflammatory cytokines levels (IL-1β, IL-12Bp40) and elevation of adiponectin (5.67±0.39 vs 2.27±0.36; P<0.001) as compared to MSG-obesity. Furthermore, significant changes were absent between alive probiotic group (VII) and intact rats (P=0.098). Single-strain analysis (group III-V) shows significant decreasing of metabolic parameters, but changes were less pronounced as compared to mixture groups and did not achieved intact rats level.

Conclusion: Multistrain formed mutualistic interactions in mixtures and therefore able to share with different metabolites, affect different receptors, which synergistic overall effect greater than the sum of the single effects.