Endocrine Abstracts

In reproductive pathology, the impact of endocrine disruptor’s on fertility potential declining is well documented; women exposed to pesticides have a risk to affect oocyte maturation and competency because Oocyte quality is a key limiting factor in female fertility which is firstly reflected by morphology feature. Centrally located cytoplasm granulation (CLCG) is one of cytoplasmic dysmoprhisms exhibited by oocytes which could be linked to pesticide exposure with relative risk to decrease ICSI outcomes. During our IVF programme we included 633 women undergoing intracytoplasmic spermatozoa injection (ICSI) program between 2009 and 2011 living in Picardy region-France with pesticide exposure. The whole lot was divided into two groups based on CLCG prevalence of oocytes (LCLCG; n=83: Low prevalence of CLCG under 25%; and HCLCG; n=68: High prevalence of CLCG over 75%). The embryological and clinical outcomes were analysed for both groups calculating the difference between them. As result, couples with HCLCG compared to LCLCG showed a decrease in embryo cleavage, ongoing pregnancy and live birth rates (82%, 14% and 13% vs 99%, 32% and 30%, respectively) while early miscarriage rate was increased (47% vs 11%) with OR (3.1 [95%CI]). Due to high pesticide exposure (over 3000 g/ha), there is higher risk to engender disturbed oocytes cohort with high prevalence of CLCG over 75%.