SFEBES2018 Poster Presentations Obesity & metabolism (24 abstracts)
Effects of visfatin on brown adipose tissue energy regulation
Georgios K Dimitriadis 1, , Raghu Adya 2 , Bee K Tan 2, , Terence A Jones 2 , Vinod S Menon 5 , Manjunath Ramanjaneya 2 , Gregory Kaltsas 2 , Alexander D Miras 6 & Harpal S Randeva 1,
1WISDEM Centre, Human Metabolism Research Unit, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, UK; 2Division of Translational and Experimental Medicine, Warwick Medical School, University of Warwick, Coventry, UK; 3Departments of Cardiovascular Sciences, Health Sciences and Leicester Diabetes Centre, College of Life Sciences, University of Leicester, Leicester, UK; 4Department of Obstetrics and Gynaecology, University Hospitals of Leicester NHS Trust, Leicester Royal Infirmary, Leicester, UK; 5Department of General Surgery, University Hospitals Coventry and Warwickshire, Coventry, UK; 6Division of Endocrinology and Experimental Medicine, Imperial College London, Hammersmith Campus, London, UK.
The role of brown adipose tissue (BAT) in pathological states of energy homeostasis and impaired adipocyte function, such as obesity has been a major area of research interest in recent years. Herein, we sought to determine the direct effects of adipokines, visfatin and leptin on BAT thermogenesis. The effects of mouse recombinant visfatin, nicotinamide mononucleotide (NMN) and leptin with or without FK866 were studied on differentiated T37i cells. Treated cells were analyzed for key genes and proteins regulating BAT [UCP-1, PRD1-BF1-RIZ1 homologous domain-containing 16 (PRDM-16), PPARgamma-coactivator-1alpha (PGC-1a) and receptor-interacting protein 140 (RIP-140)] using quantitative PCR and western blot analysis. Data is presented as mean P-values. Both visfatin and leptin had significant concentration dependent effects on thermogenesis in brown pre-adipocytes and at physiological levels, increased uncoupling protein-1 (UCP-1) levels in brown adipocytes. These effects of visfatin were similar to that of nicotinamide mononucleotide (NMN), further strengthening the enzymatic role of visfatin. We also showed that leptin induced UCP-1 mRNA expression and protein production appears to be mediated by visfatin. High concentrations of both visfatin and leptin led to a dramatic decrease in UCP-1 protein levels, supporting the notion that visfatin levels are raised in obesity and that obese people have reduced BAT activity, plausibly through a reduction in UCP-1 levels. Additionally, we found differential regulation of key brown adipogenic genes, specifically, PRD1-BF1-RIZ1 homologous domain-containing 16 (PRDM-16), PPARgamma-coactivator-1alpha (PGC-1a) and receptor-interacting protein 140 (RIP-140) by visfatin. Our observations provide novel insights in the potential actions of visfatin in BAT.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more